Biomodulative effects induced by 805 nm laser light irradiation of normal and tumor cells

M. Schaffer, R. Sroka, C. Fuchs, U. Schrader-Reichardt, P. M. Schaffer, M. Busch, E. Dühmke

Research output: Contribution to journalArticlepeer-review

36 Scopus citations


The influence of light emitted from a diode laser centred at λ = 805 nm was investigated on murine skeletal myotubes (C2), normal urothelial cells (HCV29), human squamous carcinoma cells of the gingival mucosa (ZMK) and urothelial carcinoma cells (J82) in a computer-controlled irradiation chamber. Cells were treated with varying fluences between 0 and 20 J cm-2. The response was tested by analysis of the mitotic index using single cell counting after Orcein staining and proliferation index based on BrdU incorporation during DNA synthesis. While the mitotic index of C2, HCV29 and J82 cells increased at a fluence of 4 J cm-2, irradiation with fluences of 20 J cm-2 resulted in a slight decrease. ZMK tumor cells showed a decrease of the mitotic index with both fluences. No significant differences could be determined when using irradiances between 10 mW cm-2 and 150 mW cm-2. The BrdU test after irradiation showed no significant effects compared to the controls in each cell line.

Original languageEnglish
Pages (from-to)253-257
Number of pages5
JournalJournal of Photochemistry and Photobiology B: Biology
Issue number3
StatePublished - 1 Oct 1997
Externally publishedYes


  • Biomodulative effects
  • Laser light irradiation
  • Normal cells
  • Tumor cells

ASJC Scopus subject areas

  • Radiation
  • Radiological and Ultrasound Technology
  • Biophysics
  • Radiology Nuclear Medicine and imaging


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