Biomodulative effects induced by 805 nm laser light irradiation of normal and tumor cells

M. Schaffer; R. Sroka; C. Fuchs; U. Schrader-Reichardt; P. M. Schaffer; M. Busch; E. Dühmke

doi:10.1016/S1011-1344(97)00065-1

Biomodulative effects induced by 805 nm laser light irradiation of normal and tumor cells

M. Schaffer, R. Sroka, C. Fuchs, U. Schrader-Reichardt, P. M. Schaffer, M. Busch, E. Dühmke

Research output: Contribution to journal › Article › peer-review

36 Scopus citations

Abstract

The influence of light emitted from a diode laser centred at λ = 805 nm was investigated on murine skeletal myotubes (C2), normal urothelial cells (HCV29), human squamous carcinoma cells of the gingival mucosa (ZMK) and urothelial carcinoma cells (J82) in a computer-controlled irradiation chamber. Cells were treated with varying fluences between 0 and 20 J cm^-2. The response was tested by analysis of the mitotic index using single cell counting after Orcein staining and proliferation index based on BrdU incorporation during DNA synthesis. While the mitotic index of C2, HCV29 and J82 cells increased at a fluence of 4 J cm^-2, irradiation with fluences of 20 J cm^-2 resulted in a slight decrease. ZMK tumor cells showed a decrease of the mitotic index with both fluences. No significant differences could be determined when using irradiances between 10 mW cm^-2 and 150 mW cm^-2. The BrdU test after irradiation showed no significant effects compared to the controls in each cell line.

Original language	English
Pages (from-to)	253-257
Number of pages	5
Journal	Journal of Photochemistry and Photobiology B: Biology
Volume	40
Issue number	3
DOIs	https://doi.org/10.1016/S1011-1344(97)00065-1
State	Published - 1 Oct 1997
Externally published	Yes

Keywords

Biomodulative effects
Laser light irradiation
Normal cells
Tumor cells

ASJC Scopus subject areas

Radiation
Radiological and Ultrasound Technology
Biophysics
Radiology Nuclear Medicine and imaging

Access to Document

10.1016/S1011-1344(97)00065-1

Cite this

@article{2248b0f754f34aaea612a8de6df738f0,

title = "Biomodulative effects induced by 805 nm laser light irradiation of normal and tumor cells",

abstract = "The influence of light emitted from a diode laser centred at λ = 805 nm was investigated on murine skeletal myotubes (C2), normal urothelial cells (HCV29), human squamous carcinoma cells of the gingival mucosa (ZMK) and urothelial carcinoma cells (J82) in a computer-controlled irradiation chamber. Cells were treated with varying fluences between 0 and 20 J cm-2. The response was tested by analysis of the mitotic index using single cell counting after Orcein staining and proliferation index based on BrdU incorporation during DNA synthesis. While the mitotic index of C2, HCV29 and J82 cells increased at a fluence of 4 J cm-2, irradiation with fluences of 20 J cm-2 resulted in a slight decrease. ZMK tumor cells showed a decrease of the mitotic index with both fluences. No significant differences could be determined when using irradiances between 10 mW cm-2 and 150 mW cm-2. The BrdU test after irradiation showed no significant effects compared to the controls in each cell line.",

keywords = "Biomodulative effects, Laser light irradiation, Normal cells, Tumor cells",

author = "M. Schaffer and R. Sroka and C. Fuchs and U. Schrader-Reichardt and Schaffer, {P. M.} and M. Busch and E. D{\"u}hmke",

year = "1997",

month = oct,

day = "1",

doi = "10.1016/S1011-1344(97)00065-1",

language = "English",

volume = "40",

pages = "253--257",

journal = "Journal of Photochemistry and Photobiology B: Biology",

issn = "1011-1344",

publisher = "Elsevier",

number = "3",

}

TY - JOUR

T1 - Biomodulative effects induced by 805 nm laser light irradiation of normal and tumor cells

AU - Schaffer, M.

AU - Sroka, R.

AU - Fuchs, C.

AU - Schrader-Reichardt, U.

AU - Schaffer, P. M.

AU - Busch, M.

AU - Dühmke, E.

PY - 1997/10/1

Y1 - 1997/10/1

N2 - The influence of light emitted from a diode laser centred at λ = 805 nm was investigated on murine skeletal myotubes (C2), normal urothelial cells (HCV29), human squamous carcinoma cells of the gingival mucosa (ZMK) and urothelial carcinoma cells (J82) in a computer-controlled irradiation chamber. Cells were treated with varying fluences between 0 and 20 J cm-2. The response was tested by analysis of the mitotic index using single cell counting after Orcein staining and proliferation index based on BrdU incorporation during DNA synthesis. While the mitotic index of C2, HCV29 and J82 cells increased at a fluence of 4 J cm-2, irradiation with fluences of 20 J cm-2 resulted in a slight decrease. ZMK tumor cells showed a decrease of the mitotic index with both fluences. No significant differences could be determined when using irradiances between 10 mW cm-2 and 150 mW cm-2. The BrdU test after irradiation showed no significant effects compared to the controls in each cell line.

AB - The influence of light emitted from a diode laser centred at λ = 805 nm was investigated on murine skeletal myotubes (C2), normal urothelial cells (HCV29), human squamous carcinoma cells of the gingival mucosa (ZMK) and urothelial carcinoma cells (J82) in a computer-controlled irradiation chamber. Cells were treated with varying fluences between 0 and 20 J cm-2. The response was tested by analysis of the mitotic index using single cell counting after Orcein staining and proliferation index based on BrdU incorporation during DNA synthesis. While the mitotic index of C2, HCV29 and J82 cells increased at a fluence of 4 J cm-2, irradiation with fluences of 20 J cm-2 resulted in a slight decrease. ZMK tumor cells showed a decrease of the mitotic index with both fluences. No significant differences could be determined when using irradiances between 10 mW cm-2 and 150 mW cm-2. The BrdU test after irradiation showed no significant effects compared to the controls in each cell line.

KW - Biomodulative effects

KW - Laser light irradiation

KW - Normal cells

KW - Tumor cells

UR - http://www.scopus.com/inward/record.url?scp=0030702055&partnerID=8YFLogxK

U2 - 10.1016/S1011-1344(97)00065-1

DO - 10.1016/S1011-1344(97)00065-1

M3 - Article

AN - SCOPUS:0030702055

VL - 40

SP - 253

EP - 257

JO - Journal of Photochemistry and Photobiology B: Biology

JF - Journal of Photochemistry and Photobiology B: Biology

SN - 1011-1344

IS - 3

ER -

Biomodulative effects induced by 805 nm laser light irradiation of normal and tumor cells

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this