Biotic and abiotic dynamics of a high solid-state anaerobic digestion box-type container system

Andreas Walter, Maraike Probst, Stephan Hinterberger, Horst Müller, Heribert Insam

Research output: Contribution to journalArticlepeer-review

18 Scopus citations


A solid-state anaerobic digestion box-type container system for biomethane production was observed in 12 three-week batch fermentations. Reactor performance was monitored using physico-chemical analysis and the methanogenic community was identified using ANAEROCHIP-microarrays and quantitative PCR. A resilient community was found in all batches, despite variations in inoculum to substrate ratio, feedstock quality, and fluctuating reactor conditions. The consortia were dominated by mixotrophic Methanosarcina that were accompanied by hydrogenotrophic Methanobacterium, Methanoculleus, and Methanocorpusculum. The relationship between biotic and abiotic variables was investigated using bivariate correlation analysis and univariate analysis of variance. High amounts of biogas were produced in batches with high copy numbers of Methanosarcina. High copy numbers of Methanocorpusculum and extensive percolation, however, were found to negatively correlate with biogas production. Supporting these findings, a negative correlation was detected between Methanocorpusculum and Methanosarcina. Based on these results, this study suggests Methanosarcina as an indicator for well-functioning reactor performance.

Original languageEnglish
Pages (from-to)26-35
Number of pages10
JournalWaste Management
StatePublished - 1 Mar 2016
Externally publishedYes


  • Anaerobic digestion
  • High solid-state
  • Methanogens
  • Microarray
  • Percolation
  • QPCR

ASJC Scopus subject areas

  • Waste Management and Disposal


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