Biotinylated alginate immobilization matrix in the construction of an amperometric biosensor: Application for the determination of glucose

Serge Cosnier, Andrés Novoa, Christine Mousty, Robert S. Marks

    Research output: Contribution to journalArticlepeer-review

    44 Scopus citations

    Abstract

    The use of biotinylated alginate as an immobilization matrix of enzymes on the surface of the amperometric transducer is described herein. The model used is that of the well-established glucose detection. Several types of immobilization protocols were tested. In the exception of one protocol, biotin labeled glucose oxidase was shown to first require conjugation with avidin, before its immobilization onto a biotin-alginate gel matrix. The response of the biosensors to incremental additions of glucose, was measured by potentiostating the modified electrodes at 0.6V/SCE. The permeability of the modified electrodes was thereafter measured by using rotating disk electrode (RDE) voltammetry with ferrocenemonocarboxylic acid as the electroactive probe.

    Original languageEnglish
    Pages (from-to)71-79
    Number of pages9
    JournalAnalytica Chimica Acta
    Volume453
    Issue number1
    DOIs
    StatePublished - 18 Feb 2002

    Keywords

    • Alginate
    • Avidin-biotin
    • Biosensor
    • Enzyme electrode
    • Glucose oxidase

    Fingerprint

    Dive into the research topics of 'Biotinylated alginate immobilization matrix in the construction of an amperometric biosensor: Application for the determination of glucose'. Together they form a unique fingerprint.

    Cite this