TY - JOUR
T1 - Biowaste
T2 - A Lactobacillus habitat and lactic acid fermentation substrate
AU - Probst, Maraike
AU - Fritschi, Annika
AU - Wagner, Andreas
AU - Insam, Heribert
N1 - Funding Information:
We kindly thank Jennifer Stoifl, Johannes Mair, Ursula Peintner and Frederic Gerke for their analytical help. This work was financed by the Klima- und Energiefond project “BioKasEn” in the framework of “Neue Energien 2020”, headed by Anke Bockreis. Maraike Probst was partly funded by the “Doktoratsstipendium aus der Nachwuchsförderung” of the University of Innsbruck. This work was also funded by the “Aktion D. Swarovski & Co., 2011”.
PY - 2013/1/1
Y1 - 2013/1/1
N2 - Composite organic waste was assessed for its physical, chemical and microbial suitability to serve as a substrate for the fermentative production of lactic acid. The biowaste studied was highly acidic (pH 4.3) and had high organic carbon content (45%). A clone library identified 90% of the bacterial community were lactic acid bacteria, mainly represented by Lactobacilli (70%). Cultivation using semiselective media identified Lactobacillus plantarum, Lactobacillus brevis and their closest relatives as the dominating taxa. PCR-DGGE using general bacterial and lactic acid bacterial specific primers resulted in little heterogeneity of microbial community. These data indicate that biowaste is a preferred habitat of lactic acid bacteria, suggesting that the unsterilized biowaste and its natural flora could be used in a fermentation process for lactic acid production. Such kind of biowaste application could be an alternative for current substrates and provide a modern, efficient and environmental friendly waste treatment technology.
AB - Composite organic waste was assessed for its physical, chemical and microbial suitability to serve as a substrate for the fermentative production of lactic acid. The biowaste studied was highly acidic (pH 4.3) and had high organic carbon content (45%). A clone library identified 90% of the bacterial community were lactic acid bacteria, mainly represented by Lactobacilli (70%). Cultivation using semiselective media identified Lactobacillus plantarum, Lactobacillus brevis and their closest relatives as the dominating taxa. PCR-DGGE using general bacterial and lactic acid bacterial specific primers resulted in little heterogeneity of microbial community. These data indicate that biowaste is a preferred habitat of lactic acid bacteria, suggesting that the unsterilized biowaste and its natural flora could be used in a fermentation process for lactic acid production. Such kind of biowaste application could be an alternative for current substrates and provide a modern, efficient and environmental friendly waste treatment technology.
KW - Lactic acid bacteria
KW - Municipal organic waste
KW - Organic residue
KW - Unsterilized biowaste
UR - http://www.scopus.com/inward/record.url?scp=84880633884&partnerID=8YFLogxK
U2 - 10.1016/j.biortech.2013.06.022
DO - 10.1016/j.biortech.2013.06.022
M3 - Article
AN - SCOPUS:84880633884
SN - 0960-8524
VL - 143
SP - 647
EP - 652
JO - Bioresource Technology
JF - Bioresource Technology
ER -
