Biphasic effect of sodium fluoride and guanyl nucleotides on binding to prostaglandin E2 receptors in rat epididymal adipocyte membranes

Rivka Cohen-Luria, Lioria Sigler, Gilad Rimon

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

Both NaCl and NaF promoted PGE2 binding to epididymal adipocyte membranes by apparent increase in the binding affinity. In order to distinguish between the effect of fluoride and the 'salt effect' of sodium on PGE2 binding, the effects of Mg2+ and guanyl nucleotides on PGE2 binding in the presence of NaCl or NaF were compared. Mg2+ decreased PGE2 binding; high NaF concentration abolished this inhibition, while increased NaCl concentratipns did not affect the Mg2+ inhibition. In the presence of Mg2+ the effects of NaCl and NaF were additive. The enhancement of PGE2 binding by fluoride, unlike sodium, was dependent on the presence of Mg2+. Induction of the membranes with GDPβS, Gpp(NH)p, GTP or GTPγS increased PGE, binding. Gradual increase in NaF concentrations in the presence of guanyl nucleotides resulted in stimulation of PGE2 binding at low NaF concentrations and inhibition of PGE2 binding at higjh NaF concentrations. No changes in the stimulatory action of NaCl on PGE2 binding were observed in the simulatenous presence of NaCl and guanyl nucleotides. A biphasic effect on PGE2 binding was observed with a wide concentration range of guanyl nucleotides. Treatment of the isolated membranes with cholera or pertussis toxins stimulated the adenylyl cyclase activity of the membranes, but failed to influence PGE2 binding. The implications of these findings are discussed.

Original languageEnglish
Pages (from-to)561-568
Number of pages8
JournalCellular Signalling
Volume1
Issue number6
DOIs
StatePublished - 1 Jan 1989

Keywords

  • Prostaglandin receptor
  • epididymal adipocyte membrane
  • guanyl nucleotides
  • sodium fluoride

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