Blotting from PhastGel to membranes by ultrasound.

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3 Scopus citations

Abstract

Ultrasound-based approach for enhanced protein blotting is proposed. Three minutes of ultrasound exposure (1 MHz, 2.5 W/cm(2)) was sufficient for a clear transfer of proteins from a polyacrylamide gel (PhastGel) to nitrocellulose or Nylon 66 Biotrans membrane. The proteins evaluated were prestained sodium dodecyl sulfate-polyacrylamide standards (18,500-106,000 Da) and (14)C-labeled Rainbow protein molecular weight markers (14,300-200,000 Da).

Original languageEnglish
Pages (from-to)173-179
Number of pages7
JournalMethods in Molecular Biology
Volume536
DOIs
StatePublished - 1 Jan 2009

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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