Blotting from phastgel to membranes by ultrasound

Joseph Kost, Aharon Azagury

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

1 Scopus citations


Ultrasound based approach for enhanced protein blotting is proposed. Three minutes of ultrasound exposure (1 MHz, 2.5 W/cm 2) was sufficient for a clear transfer of proteins from a polyacrylamide gel (PhastGel) to nitrocellulose or Nylon 66 Biotrans membrane. The proteins evaluated were prestained sodium dodecyl sulfate-polyacrylamide standards (18,500-106,000 Da) and 14C-labeled Rainbow protein molecular weight markers (14,300-200,000 Da).

Original languageEnglish
Title of host publicationWestern Blotting
Subtitle of host publicationMethods and Protocols
PublisherSpringer New York
Number of pages10
ISBN (Electronic)9781493926947
ISBN (Print)9781493926930
StatePublished - 4 Jun 2015


  • Mass transfer
  • Nitrocellulose membranes
  • Nylon 66 biotrans membranes
  • Phastgel
  • Polyacrylamide gel
  • Protein blotting
  • Ultrasound

ASJC Scopus subject areas

  • General Biochemistry, Genetics and Molecular Biology
  • General Medicine


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