Abstract
Ultrasound based approach for enhanced protein blotting is proposed. Three minutes of ultrasound exposure (1 MHz, 2.5 W/cm 2) was sufficient for a clear transfer of proteins from a polyacrylamide gel (PhastGel) to nitrocellulose or Nylon 66 Biotrans membrane. The proteins evaluated were prestained sodium dodecyl sulfate-polyacrylamide standards (18,500-106,000 Da) and 14C-labeled Rainbow protein molecular weight markers (14,300-200,000 Da).
Original language | English |
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Title of host publication | Western Blotting |
Subtitle of host publication | Methods and Protocols |
Publisher | Springer New York |
Pages | 237-246 |
Number of pages | 10 |
ISBN (Electronic) | 9781493926947 |
ISBN (Print) | 9781493926930 |
DOIs | |
State | Published - 4 Jun 2015 |
Keywords
- Mass transfer
- Nitrocellulose membranes
- Nylon 66 biotrans membranes
- Phastgel
- Polyacrylamide gel
- Protein blotting
- Ultrasound
ASJC Scopus subject areas
- General Biochemistry, Genetics and Molecular Biology
- General Medicine