Blotting from PhastGel to membranes by ultrasound

Joseph Kost; Aharon Azagury

doi:10.1007/978-1-4939-2694-7_25

Blotting from PhastGel to membranes by ultrasound

Joseph Kost, Aharon Azagury

Department of Chemical Engineering

Research output: Contribution to journal › Article › peer-review

Abstract

Ultrasound based approach for enhanced protein blotting is proposed. Three minutes of ultrasound exposure (1 MHz, 2.5 W/cm²) was suffi cient for a clear transfer of proteins from a polyacrylamide gel (PhastGel) to nitrocellulose or Nylon 66 Biotrans membrane. The proteins evaluated were prestained sodium dodecyl sulfate-polyacrylamide standards (18,500–106,000 Da) and 14C-labeled Rainbow protein molecular weight markers (14,300–200,000 Da).

Original language	English
Pages (from-to)	237-246
Number of pages	10
Journal	Methods in Molecular Biology
Volume	1312
DOIs	https://doi.org/10.1007/978-1-4939-2694-7_25
State	Published - 1 Jan 2015

Keywords

Mass transfer
Nitrocellulose membranes
Nylon 66 biotrans membranes
PhastGel
Polyacrylamide gel
Protein blotting
Ultrasound

ASJC Scopus subject areas

Molecular Biology
Genetics

Access to Document

10.1007/978-1-4939-2694-7_25

Cite this

@article{009dfa9cd3a94404a64a0a785d6a3d3c,

title = "Blotting from PhastGel to membranes by ultrasound",

abstract = "Ultrasound based approach for enhanced protein blotting is proposed. Three minutes of ultrasound exposure (1 MHz, 2.5 W/cm2) was suffi cient for a clear transfer of proteins from a polyacrylamide gel (PhastGel) to nitrocellulose or Nylon 66 Biotrans membrane. The proteins evaluated were prestained sodium dodecyl sulfate-polyacrylamide standards (18,500–106,000 Da) and 14C-labeled Rainbow protein molecular weight markers (14,300–200,000 Da).",

keywords = "Mass transfer, Nitrocellulose membranes, Nylon 66 biotrans membranes, PhastGel, Polyacrylamide gel, Protein blotting, Ultrasound",

author = "Joseph Kost and Aharon Azagury",

note = "Publisher Copyright: {\textcopyright} Springer Science+Business Media New York 2015.",

year = "2015",

month = jan,

day = "1",

doi = "10.1007/978-1-4939-2694-7_25",

language = "English",

volume = "1312",

pages = "237--246",

journal = "Methods in Molecular Biology",

issn = "1064-3745",

publisher = "Humana Press Inc.",

}

TY - JOUR

T1 - Blotting from PhastGel to membranes by ultrasound

AU - Kost, Joseph

AU - Azagury, Aharon

N1 - Publisher Copyright: © Springer Science+Business Media New York 2015.

PY - 2015/1/1

Y1 - 2015/1/1

N2 - Ultrasound based approach for enhanced protein blotting is proposed. Three minutes of ultrasound exposure (1 MHz, 2.5 W/cm2) was suffi cient for a clear transfer of proteins from a polyacrylamide gel (PhastGel) to nitrocellulose or Nylon 66 Biotrans membrane. The proteins evaluated were prestained sodium dodecyl sulfate-polyacrylamide standards (18,500–106,000 Da) and 14C-labeled Rainbow protein molecular weight markers (14,300–200,000 Da).

AB - Ultrasound based approach for enhanced protein blotting is proposed. Three minutes of ultrasound exposure (1 MHz, 2.5 W/cm2) was suffi cient for a clear transfer of proteins from a polyacrylamide gel (PhastGel) to nitrocellulose or Nylon 66 Biotrans membrane. The proteins evaluated were prestained sodium dodecyl sulfate-polyacrylamide standards (18,500–106,000 Da) and 14C-labeled Rainbow protein molecular weight markers (14,300–200,000 Da).

KW - Mass transfer

KW - Nitrocellulose membranes

KW - Nylon 66 biotrans membranes

KW - PhastGel

KW - Polyacrylamide gel

KW - Protein blotting

KW - Ultrasound

UR - http://www.scopus.com/inward/record.url?scp=84930959007&partnerID=8YFLogxK

U2 - 10.1007/978-1-4939-2694-7_25

DO - 10.1007/978-1-4939-2694-7_25

M3 - Article

AN - SCOPUS:84930959007

SN - 1064-3745

VL - 1312

SP - 237

EP - 246

JO - Methods in Molecular Biology

JF - Methods in Molecular Biology

ER -

Blotting from PhastGel to membranes by ultrasound

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this