Blotting from PhastGel to membranes by ultrasound.

Joseph Kost

doi:10.1007/978-1-59745-542-8_19

Blotting from PhastGel to membranes by ultrasound.

Joseph Kost

Department of Chemical Engineering

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

Ultrasound-based approach for enhanced protein blotting is proposed. Three minutes of ultrasound exposure (1 MHz, 2.5 W/cm(2)) was sufficient for a clear transfer of proteins from a polyacrylamide gel (PhastGel) to nitrocellulose or Nylon 66 Biotrans membrane. The proteins evaluated were prestained sodium dodecyl sulfate-polyacrylamide standards (18,500-106,000 Da) and (14)C-labeled Rainbow protein molecular weight markers (14,300-200,000 Da).

Original language	English
Pages (from-to)	173-179
Number of pages	7
Journal	Methods in molecular biology (Clifton, N.J.)
Volume	536
DOIs	https://doi.org/10.1007/978-1-59745-542-8_19
State	Published - 1 Jan 2009

ASJC Scopus subject areas

Molecular Biology
Genetics

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10.1007/978-1-59745-542-8_19

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abstract = "Ultrasound-based approach for enhanced protein blotting is proposed. Three minutes of ultrasound exposure (1 MHz, 2.5 W/cm(2)) was sufficient for a clear transfer of proteins from a polyacrylamide gel (PhastGel) to nitrocellulose or Nylon 66 Biotrans membrane. The proteins evaluated were prestained sodium dodecyl sulfate-polyacrylamide standards (18,500-106,000 Da) and (14)C-labeled Rainbow protein molecular weight markers (14,300-200,000 Da).",

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AB - Ultrasound-based approach for enhanced protein blotting is proposed. Three minutes of ultrasound exposure (1 MHz, 2.5 W/cm(2)) was sufficient for a clear transfer of proteins from a polyacrylamide gel (PhastGel) to nitrocellulose or Nylon 66 Biotrans membrane. The proteins evaluated were prestained sodium dodecyl sulfate-polyacrylamide standards (18,500-106,000 Da) and (14)C-labeled Rainbow protein molecular weight markers (14,300-200,000 Da).

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Blotting from PhastGel to membranes by ultrasound.

Abstract

ASJC Scopus subject areas

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