TY - JOUR
T1 - Bolaamphiphilic vesicles encapsulating iron oxide nanoparticles
T2 - New vehicles for magnetically targeted drug delivery
AU - Philosof-Mazor, Liron
AU - Dakwar, George R.
AU - Popov, Mary
AU - Kolusheva, Sofiya
AU - Shames, Alexander
AU - Linder, Charles
AU - Greenberg, Sarina
AU - Heldman, Eliahu
AU - Stepensky, David
AU - Jelinek, Raz
N1 - Funding Information:
We thank Prof. Philip Lazarovici (Institute for Drug Research, School of Pharmacy, The Hebrew University of Jerusalem, Israel) for providing the b.End3 cells. This study was supported by the Israel Science Foundation Grant No. 973/11 to David Stepensky, Eli Heldman, Sarina Grinberg and Charles Linder.
PY - 2013/6/25
Y1 - 2013/6/25
N2 - Bolaamphiphiles - amphiphilic molecules consisting of two hydrophilic headgroups linked by a hydrophobic chain - form highly stable vesicles consisting of a monolayer membrane that can be used as vehicles to deliver drugs across biological membranes, particularly the blood-brain barrier (BBB). We prepared new vesicles comprising bolaamphiphiles (bolavesicles) that encapsulate iron oxide nanoparticles (IONPs) and investigated their suitability for targeted drug delivery. Bolavesicles displaying different headgroups were studied, and the effect of IONP encapsulation upon membrane interactions and cell uptake were examined. Experiments revealed more pronounced membrane interactions of the bolavesicles assembled with IONPs. Furthermore, enhanced internalization and stability of the IONP-bolavesicles were observed in b.End3 brain microvessel endothelial cells - an in vitro model of the blood-brain barrier. Our findings indicate that embedded IONPs modulate bolavesicles' physicochemical properties, endow higher vesicle stability, and enhance their membrane permeability and cellular uptake. IONP-bolavesicles thus constitute a promising drug delivery platform, potentially targeted to the desired location using external magnetic field.
AB - Bolaamphiphiles - amphiphilic molecules consisting of two hydrophilic headgroups linked by a hydrophobic chain - form highly stable vesicles consisting of a monolayer membrane that can be used as vehicles to deliver drugs across biological membranes, particularly the blood-brain barrier (BBB). We prepared new vesicles comprising bolaamphiphiles (bolavesicles) that encapsulate iron oxide nanoparticles (IONPs) and investigated their suitability for targeted drug delivery. Bolavesicles displaying different headgroups were studied, and the effect of IONP encapsulation upon membrane interactions and cell uptake were examined. Experiments revealed more pronounced membrane interactions of the bolavesicles assembled with IONPs. Furthermore, enhanced internalization and stability of the IONP-bolavesicles were observed in b.End3 brain microvessel endothelial cells - an in vitro model of the blood-brain barrier. Our findings indicate that embedded IONPs modulate bolavesicles' physicochemical properties, endow higher vesicle stability, and enhance their membrane permeability and cellular uptake. IONP-bolavesicles thus constitute a promising drug delivery platform, potentially targeted to the desired location using external magnetic field.
KW - Bolaamphiphiles
KW - Iron oxide nanoparticles
KW - Magnetic nanoparticles
KW - Nano-drug delivery systems
KW - Targeted drug delivery
UR - http://www.scopus.com/inward/record.url?scp=84877840124&partnerID=8YFLogxK
U2 - 10.1016/j.ijpharm.2013.04.017
DO - 10.1016/j.ijpharm.2013.04.017
M3 - Article
C2 - 23623794
AN - SCOPUS:84877840124
SN - 0378-5173
VL - 450
SP - 241
EP - 249
JO - International Journal of Pharmaceutics
JF - International Journal of Pharmaceutics
IS - 1-2
ER -