@inproceedings{f8c45a0fef3843f9b6c31c244d3b934b,
title = "Braincubator: An incubation system to extend brain slice lifespan for use in neurophysiology",
abstract = "In vitro brain slice preparations are instrumental in developing our understanding of the nervous system. However, the current lifespan of an acute brain slice is limited to approximately 6-12 hours. This reduces potential experimentation time and leads to considerable waste of neural tissue. We have designed, developed and tested a novel incubation system capable of extending the lifespan of these brain slices. This is done by controlling the temperature and pH of the artificial cerebral spinal fluid in which the slices are incubated while continuously passing the fluid through a UVC filtration system. This system is capable of maintaining extremely low bacterial levels and significantly extending the brain slice lifespan to at least 24 hours. Brain slice viability was validated through electrophysiological recordings as well as live/dead cell assays.",
author = "Breen, \{Paul P.\} and Yossi Buskila",
note = "Publisher Copyright: {\textcopyright} 2014 IEEE.; 2014 36th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2014 ; Conference date: 26-08-2014 Through 30-08-2014",
year = "2014",
month = nov,
day = "2",
doi = "10.1109/EMBC.2014.6944713",
language = "English",
series = "2014 36th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2014",
publisher = "Institute of Electrical and Electronics Engineers",
pages = "4864--4867",
booktitle = "2014 36th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2014",
address = "United States",
}