Breast biopsy nuclear pellets are a convenient source of DNA for routine determination of Her-2/neu gene amplification

Ofra Malka; Yaakov Pollack; Daniel Benharroch; Yoram Cohen; Ron Levy; Ilana Yanai-Inbar; Jacob Gopas

doi:10.1007/BF01975205

Breast biopsy nuclear pellets are a convenient source of DNA for routine determination of Her-2/neu gene amplification

Ofra Malka, Yaakov Pollack, Daniel Benharroch, Yoram Cohen, Ron Levy, Ilana Yanai-Inbar, Jacob Gopas

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

Cell nuclei from breast biopsies are a byproduct of routine hormone receptor determination and are usually discarded. We report here that DNA purified from this source is suitable for use in conventional Southern blot or Polymerase Chain Reaction (PCR) techniques to screen for Her-2/neu amplification, enabling efficient use of limited biopsy material.

Original language	English
Pages (from-to)	57-61
Number of pages	5
Journal	Breast Cancer Research and Treatment
Volume	19
Issue number	1
DOIs	https://doi.org/10.1007/BF01975205
State	Published - 1 Sep 1991

Keywords

breast biopsy
Her-2/neu gene amplification
polymerase chain reaction
Southern blot

ASJC Scopus subject areas

Oncology
Cancer Research

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1007/BF01975205

Cite this

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abstract = "Cell nuclei from breast biopsies are a byproduct of routine hormone receptor determination and are usually discarded. We report here that DNA purified from this source is suitable for use in conventional Southern blot or Polymerase Chain Reaction (PCR) techniques to screen for Her-2/neu amplification, enabling efficient use of limited biopsy material.",

keywords = "breast biopsy, Her-2/neu gene amplification, polymerase chain reaction, Southern blot",

author = "Ofra Malka and Yaakov Pollack and Daniel Benharroch and Yoram Cohen and Ron Levy and Ilana Yanai-Inbar and Jacob Gopas",

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T1 - Breast biopsy nuclear pellets are a convenient source of DNA for routine determination of Her-2/neu gene amplification

AU - Malka, Ofra

AU - Pollack, Yaakov

AU - Benharroch, Daniel

AU - Cohen, Yoram

AU - Levy, Ron

AU - Yanai-Inbar, Ilana

AU - Gopas, Jacob

PY - 1991/9/1

Y1 - 1991/9/1

N2 - Cell nuclei from breast biopsies are a byproduct of routine hormone receptor determination and are usually discarded. We report here that DNA purified from this source is suitable for use in conventional Southern blot or Polymerase Chain Reaction (PCR) techniques to screen for Her-2/neu amplification, enabling efficient use of limited biopsy material.

AB - Cell nuclei from breast biopsies are a byproduct of routine hormone receptor determination and are usually discarded. We report here that DNA purified from this source is suitable for use in conventional Southern blot or Polymerase Chain Reaction (PCR) techniques to screen for Her-2/neu amplification, enabling efficient use of limited biopsy material.

KW - breast biopsy

KW - Her-2/neu gene amplification

KW - polymerase chain reaction

KW - Southern blot

UR - http://www.scopus.com/inward/record.url?scp=0025989227&partnerID=8YFLogxK

U2 - 10.1007/BF01975205

DO - 10.1007/BF01975205

M3 - Article

C2 - 1684521

AN - SCOPUS:0025989227

SN - 0167-6806

VL - 19

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JO - Breast Cancer Research and Treatment

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IS - 1

ER -

Breast biopsy nuclear pellets are a convenient source of DNA for routine determination of Her-2/neu gene amplification

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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