Brownian Dynamics Simulation of FCS Measurements on Single Fluorophore-Labeled Superhelical DNA

Jan Krieger, Tomasz Wocjan, Oleg Krichevsky, Jörg Langowski

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We investigated the dynamics of a single-fluorophore-labeled pUC18 plasmid through a Brownian dynamics algorithm, followed up by a simulation of the Fluorescence Correlation Spectroscopy (FCS) process. Recent experimental FCS measurements indicated a sensitivity of the monomer mean square displacements in DNA circles towards superhelicity. Simulations with homogeneous DNA elasticity and local straight equilibrium are not sufficient to reproduce this observed behavior. But inserting permanently bent sequences into the DNA, which favor end loop formation, caused a dependence of the calculated FCS correlation curves on superhelical density. Furthermore, our simulations allow us to take into account the orientation of the fluorophore in polarized excitation, which might explain the observed appearance of a Rouse-like regime at intermediate time scales.
Original languageEnglish
Pages (from-to)570a
JournalBiophysical Journal
Issue number3
StatePublished - Jan 2010

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