Bubble Dynamics in Double-Stranded DNA

Grégoire Altan-Bonnet; Albert Libchaber; Oleg Krichevsky

doi:10.1103/PhysRevLett.90.138101

Bubble Dynamics in Double-Stranded DNA

Grégoire Altan-Bonnet, Albert Libchaber, Oleg Krichevsky

Department of Physics

Research output: Contribution to journal › Article › peer-review

28 Scopus citations

Abstract

We report the first measurement of the dynamics of bubble formation in double-stranded DNA. Fluctuations of fluorescence of a synthetic DNA construct, internally tagged with a fluorophore and a quencher, are monitored by fluorescence correlation spectroscopy. The relaxation dynamics follow a multistate relaxation kinetics, with a characteristic time scale of [Formula presented]. A simple model of bubble dynamics based on constant zipping-unzipping rates is proposed to account for our experimental data. The role of different secondary structures stabilizing the open bubble is tested.

Original language	English
Pages (from-to)	4
Number of pages	1
Journal	Physical Review Letters
Volume	90
Issue number	13
DOIs	https://doi.org/10.1103/PhysRevLett.90.138101
State	Published - 1 Jan 2003

ASJC Scopus subject areas

General Physics and Astronomy

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10.1103/PhysRevLett.90.138101

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title = "Bubble Dynamics in Double-Stranded DNA",

abstract = "We report the first measurement of the dynamics of bubble formation in double-stranded DNA. Fluctuations of fluorescence of a synthetic DNA construct, internally tagged with a fluorophore and a quencher, are monitored by fluorescence correlation spectroscopy. The relaxation dynamics follow a multistate relaxation kinetics, with a characteristic time scale of [Formula presented]. A simple model of bubble dynamics based on constant zipping-unzipping rates is proposed to account for our experimental data. The role of different secondary structures stabilizing the open bubble is tested.",

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AU - Krichevsky, Oleg

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N2 - We report the first measurement of the dynamics of bubble formation in double-stranded DNA. Fluctuations of fluorescence of a synthetic DNA construct, internally tagged with a fluorophore and a quencher, are monitored by fluorescence correlation spectroscopy. The relaxation dynamics follow a multistate relaxation kinetics, with a characteristic time scale of [Formula presented]. A simple model of bubble dynamics based on constant zipping-unzipping rates is proposed to account for our experimental data. The role of different secondary structures stabilizing the open bubble is tested.

AB - We report the first measurement of the dynamics of bubble formation in double-stranded DNA. Fluctuations of fluorescence of a synthetic DNA construct, internally tagged with a fluorophore and a quencher, are monitored by fluorescence correlation spectroscopy. The relaxation dynamics follow a multistate relaxation kinetics, with a characteristic time scale of [Formula presented]. A simple model of bubble dynamics based on constant zipping-unzipping rates is proposed to account for our experimental data. The role of different secondary structures stabilizing the open bubble is tested.

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DO - 10.1103/PhysRevLett.90.138101

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JO - Physical Review Letters

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Bubble Dynamics in Double-Stranded DNA

Abstract

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