TY - JOUR
T1 - Calcium-siRNA nanocomplexes
T2 - What reversibility is all about
AU - Ruvinov, Emil
AU - Kryukov, Olga
AU - Forti, Efrat
AU - Korin, Efrat
AU - Goldstein, Matan
AU - Cohen, Smadar
N1 - Publisher Copyright:
© 2015 Elsevier B.V. All rights reserved.
PY - 2015/4/10
Y1 - 2015/4/10
N2 - Gene silencing using small interfering RNA (siRNA) relies on the critical need for a safe and effective carrier, capable of strong but reversible complexation, siRNA protection, cellular uptake, and cytoplasmatic unloading of its cargo. We hypothesized that a delivery platform based on the eletrostatic interactions of siRNA with calcium ions in solution would fulfill these needs, ultimately leading to effective gene silencing. Physical characterization of the calcium-siRNA complexes, using high resolution microscopy and dynamic light scattering (DLS), showed the formation of stable nanosized complexes ~ 80 nm in diameter, bearing mild (~ - 7 mV) negative surface charge. The complexes were extremely stable in the presence of serum proteins or high concentrations of heparin; they maintained their nanosized features in suspension for days; and effectively protected the siRNA from enzymatic degradation. The Ca-siRNA complexes were disintegrated in the presence of Ca-chelating ion exchange resin, thus proving their reversibility. Excellent cytocompatibility of calcium-siRNA complexes was achieved using physiological calcium ion concentrations. The calcium-siRNA complexes successfully induced a very high (~ 80%) level of gene silencing in several cell types, at both mRNA and protein levels, associated with efficient cellular uptake. Collectively, our results show that the developed delivery platform based on reversible calcium-siRNA interactions offers a simple and versatile method for enhancing the therapeutic efficiency of siRNA.
AB - Gene silencing using small interfering RNA (siRNA) relies on the critical need for a safe and effective carrier, capable of strong but reversible complexation, siRNA protection, cellular uptake, and cytoplasmatic unloading of its cargo. We hypothesized that a delivery platform based on the eletrostatic interactions of siRNA with calcium ions in solution would fulfill these needs, ultimately leading to effective gene silencing. Physical characterization of the calcium-siRNA complexes, using high resolution microscopy and dynamic light scattering (DLS), showed the formation of stable nanosized complexes ~ 80 nm in diameter, bearing mild (~ - 7 mV) negative surface charge. The complexes were extremely stable in the presence of serum proteins or high concentrations of heparin; they maintained their nanosized features in suspension for days; and effectively protected the siRNA from enzymatic degradation. The Ca-siRNA complexes were disintegrated in the presence of Ca-chelating ion exchange resin, thus proving their reversibility. Excellent cytocompatibility of calcium-siRNA complexes was achieved using physiological calcium ion concentrations. The calcium-siRNA complexes successfully induced a very high (~ 80%) level of gene silencing in several cell types, at both mRNA and protein levels, associated with efficient cellular uptake. Collectively, our results show that the developed delivery platform based on reversible calcium-siRNA interactions offers a simple and versatile method for enhancing the therapeutic efficiency of siRNA.
KW - Calcium complex
KW - Drug delivery
KW - Gene silencing
KW - RNA interference
KW - Transfection
KW - siRNA delivery
UR - http://www.scopus.com/inward/record.url?scp=84923362767&partnerID=8YFLogxK
U2 - 10.1016/j.jconrel.2015.02.029
DO - 10.1016/j.jconrel.2015.02.029
M3 - Article
C2 - 25702963
AN - SCOPUS:84923362767
SN - 0168-3659
VL - 203
SP - 150
EP - 160
JO - Journal of Controlled Release
JF - Journal of Controlled Release
ER -