TY - JOUR
T1 - CAMTA transcription factor enhances salinity and drought tolerance in chickpea (Cicer arietinum L.)
AU - Meenakshi,
AU - Kumar, Anil
AU - Kumar, Varun
AU - Dubey, Arvind Kumar
AU - Narayan, Shiv
AU - Sawant, Samir V.
AU - Pande, Veena
AU - Shirke, Pramod Arvind
AU - Sanyal, Indraneel
N1 - Publisher Copyright:
© 2021, The Author(s), under exclusive licence to Springer Nature B.V.
PY - 2022/2/1
Y1 - 2022/2/1
N2 - Various abiotic stresses like drought, salinity, high temperature, and chilling adversely affect plant growth and productivity. Terminal drought stress is one of the major concerns which limits the growth and yield of chickpeas. Calmodulin binding transcription activator (CAMTA) plays a vital role in stress tolerance in plants. In this study, the role of the CAMTA gene was assessed by over-expression in chickpea (Cicer arietinum L.) in response to drought and salinity stress. The over-expressing lines of the CAMTA gene have shown enhanced activities of various antioxidant enzymes (ascorbate peroxidase (APX), catalase (CAT), glutathione S-transferase (GST), superoxide dismutase (SOD), monodehydroascorbate reductase (MDHAR)). The reduced stress markers TBARS and H2O2 enhanced the survival of plants against both stresses. The physiological parameters (net photosynthesis; PN, transpiration; E, stomatal conductance; gs, photochemical quenching; qP, non-photochemical quenching; qN, and electron transport rate; ETR) were improved in the transgenics under both the stresses that protected the plants from damage. This investigation verified that the CAMTA gene provides tolerance against drought and salinity by maintaining biochemical, physiological, and morphological performances and could be exploited for genetic engineering strategies to overcome the stresses in other economically important crops.
AB - Various abiotic stresses like drought, salinity, high temperature, and chilling adversely affect plant growth and productivity. Terminal drought stress is one of the major concerns which limits the growth and yield of chickpeas. Calmodulin binding transcription activator (CAMTA) plays a vital role in stress tolerance in plants. In this study, the role of the CAMTA gene was assessed by over-expression in chickpea (Cicer arietinum L.) in response to drought and salinity stress. The over-expressing lines of the CAMTA gene have shown enhanced activities of various antioxidant enzymes (ascorbate peroxidase (APX), catalase (CAT), glutathione S-transferase (GST), superoxide dismutase (SOD), monodehydroascorbate reductase (MDHAR)). The reduced stress markers TBARS and H2O2 enhanced the survival of plants against both stresses. The physiological parameters (net photosynthesis; PN, transpiration; E, stomatal conductance; gs, photochemical quenching; qP, non-photochemical quenching; qN, and electron transport rate; ETR) were improved in the transgenics under both the stresses that protected the plants from damage. This investigation verified that the CAMTA gene provides tolerance against drought and salinity by maintaining biochemical, physiological, and morphological performances and could be exploited for genetic engineering strategies to overcome the stresses in other economically important crops.
KW - Agrobacterium-mediated transformation
KW - Antioxidants
KW - CAMTA
KW - Cicer arietinum (L.)
KW - Drought
UR - http://www.scopus.com/inward/record.url?scp=85118557777&partnerID=8YFLogxK
U2 - 10.1007/s11240-021-02191-3
DO - 10.1007/s11240-021-02191-3
M3 - Article
AN - SCOPUS:85118557777
SN - 0167-6857
VL - 148
SP - 319
EP - 330
JO - Plant Cell, Tissue and Organ Culture
JF - Plant Cell, Tissue and Organ Culture
IS - 2
ER -