TY - JOUR
T1 - Cancerous glucose metabolism in lung cancer - Evidence from exhaled breath analysis
AU - Feinberg, Tali
AU - Alkoby-Meshulam, Layah
AU - Herbig, Jens
AU - Cancilla, John C.
AU - Torrecilla, Jose S.
AU - Gai Mor, Naomi
AU - Bar, Jair
AU - Ilouze, Maya
AU - Haick, Hossam
AU - Peled, Nir
N1 - Publisher Copyright:
© 2016 IOP Publishing Ltd.
PY - 2016/6/7
Y1 - 2016/6/7
N2 - Cancer cells prefer hyperglycolysis versus oxidative phosphorylation, even in the presence of oxygen. This phenomenon is used through the FDG-PET scans, and may affect the exhaled volatile signature. This study investigates the volatile signature in lung cancer (LC) before and after an oral glucose tolerance test (OGTT) to determine if tumor cells' hyperglycolysis would affect the volatile signature. Blood glucose levels and exhaled breath samples were analyzed before the OGTT, and 90 min after, in both LC patients and controls. The volatile signature was measured by proton transfer reaction mass spectrometry (PTR-MS). Twenty-two LC patients (age 66.6 ± 12.7) with adenocarcinoma (n = 14), squamous (n = 6), small cell carcinoma (n = 2), and twenty-one controls (age 54.4 ± 13.7; 10 non-smokers and 11 smokers) were included. All LC patients showed a hyperglycolytic state in their FDG-PET scans. Both baseline and post OGTT volatile signatures discriminate between the groups. The OGTT has a minimal effect in LC (a decrease in m/z 54 by 39%, p v = 0.0499); whereas in the control group, five masses (m/z 64, 87,88, 142 and 161) changed by -13%, -49%, -40% and -29% and 46% respectively. To conclude, OGTT has a minimal effect on the VOC signature in LC patients, where a hyperglycolytic state already exists. In contrast, in the control group the OGTT has a profound effect in which induced hyperglycolysis significantly changed the VOC pattern. We hypothesized that a ceiling effect in cancerous patients is responsible for this discrepancy.
AB - Cancer cells prefer hyperglycolysis versus oxidative phosphorylation, even in the presence of oxygen. This phenomenon is used through the FDG-PET scans, and may affect the exhaled volatile signature. This study investigates the volatile signature in lung cancer (LC) before and after an oral glucose tolerance test (OGTT) to determine if tumor cells' hyperglycolysis would affect the volatile signature. Blood glucose levels and exhaled breath samples were analyzed before the OGTT, and 90 min after, in both LC patients and controls. The volatile signature was measured by proton transfer reaction mass spectrometry (PTR-MS). Twenty-two LC patients (age 66.6 ± 12.7) with adenocarcinoma (n = 14), squamous (n = 6), small cell carcinoma (n = 2), and twenty-one controls (age 54.4 ± 13.7; 10 non-smokers and 11 smokers) were included. All LC patients showed a hyperglycolytic state in their FDG-PET scans. Both baseline and post OGTT volatile signatures discriminate between the groups. The OGTT has a minimal effect in LC (a decrease in m/z 54 by 39%, p v = 0.0499); whereas in the control group, five masses (m/z 64, 87,88, 142 and 161) changed by -13%, -49%, -40% and -29% and 46% respectively. To conclude, OGTT has a minimal effect on the VOC signature in LC patients, where a hyperglycolytic state already exists. In contrast, in the control group the OGTT has a profound effect in which induced hyperglycolysis significantly changed the VOC pattern. We hypothesized that a ceiling effect in cancerous patients is responsible for this discrepancy.
KW - cancer metabolism
KW - hyperglycolysis
KW - lung cancer
KW - oral glucose tolerance test (OGTT)
KW - proton transfer reaction mass spectrometry (PTR-MS)
KW - volatile organic compounds (VOCs)
UR - http://www.scopus.com/inward/record.url?scp=84978758108&partnerID=8YFLogxK
U2 - 10.1088/1752-7155/10/2/026012
DO - 10.1088/1752-7155/10/2/026012
M3 - Article
C2 - 27272440
AN - SCOPUS:84978758108
SN - 1752-7155
VL - 10
JO - Journal of Breath Research
JF - Journal of Breath Research
IS - 2
M1 - 026012
ER -