TY - JOUR
T1 - Carotenoid-to-chlorophyll ratio as a proxy for assay of total fatty acids and arachidonic acid content in the green microalga Parietochloris incisa
AU - Solovchenko, A. E.
AU - Khozin-Goldberg, I.
AU - Cohen, Z.
AU - Merzlyak, M. N.
N1 - Funding Information:
Acknowledgements This work was supported in part by fellowships from the Blaustein Center for Scientific Cooperation (BCSC) to A.E.S. and M.N.M. The financial support from the Russian Foundation for Basic Research (Grant # 06-04-48883) and the Russian President’s Grant Council (Ministry of Science of the Russian Federation, Grant # MK-3433.2008.4) is also acknowledged.
PY - 2009/6/1
Y1 - 2009/6/1
N2 - The relationships between pigment (carotenoid and chlorophyll) content with accumulation of total fatty acids (TFA) and arachidonic acid (AA) were studied in the green microalga Parietochloris incisa (Trebouxiophyceae, Chlorophyta) grown under different PFDs (35, 200, and 400 μmol photons m-2 s-1) and nitrogen availabilities. The growth of P. incisa under higher light and nitrogen deficiency was accompanied by accumulation of FA, an increase in carotenoid and a decline in chlorophyll content. It was found that the carotenoid-to-chlorophyll ratio (but not the individual pigment content) correlates closely with the volumetric content of both TFA and AA. Analysis of scattering-compensated absorption spectra of P. incisa suspensions revealed their tight relationship in the blue-green range of the spectrum with the carotenoid-to-chlorophyll ratio, TFA, and AA content. These findings allowed the development of algorithms for the non-destructive assay of TFA and AA in cell suspensions in the ranges of 0.09-3.04 and 0.04-1.7 μg mL-1, with accuracy of 0.06 and 0.01 μg mL-1, respectively, via analytically measured carotenoid-to-chlorophyll ratio and using the ratio of absorption coefficients at 510 and 678 nm, with accuracy of 0.07 and 0.02 μg mL -1, respectively. The feasibility of obtaining essential spectral information concerning the physiological condition of P. incisa using a standard spectrophotometer is also shown.
AB - The relationships between pigment (carotenoid and chlorophyll) content with accumulation of total fatty acids (TFA) and arachidonic acid (AA) were studied in the green microalga Parietochloris incisa (Trebouxiophyceae, Chlorophyta) grown under different PFDs (35, 200, and 400 μmol photons m-2 s-1) and nitrogen availabilities. The growth of P. incisa under higher light and nitrogen deficiency was accompanied by accumulation of FA, an increase in carotenoid and a decline in chlorophyll content. It was found that the carotenoid-to-chlorophyll ratio (but not the individual pigment content) correlates closely with the volumetric content of both TFA and AA. Analysis of scattering-compensated absorption spectra of P. incisa suspensions revealed their tight relationship in the blue-green range of the spectrum with the carotenoid-to-chlorophyll ratio, TFA, and AA content. These findings allowed the development of algorithms for the non-destructive assay of TFA and AA in cell suspensions in the ranges of 0.09-3.04 and 0.04-1.7 μg mL-1, with accuracy of 0.06 and 0.01 μg mL-1, respectively, via analytically measured carotenoid-to-chlorophyll ratio and using the ratio of absorption coefficients at 510 and 678 nm, with accuracy of 0.07 and 0.02 μg mL -1, respectively. The feasibility of obtaining essential spectral information concerning the physiological condition of P. incisa using a standard spectrophotometer is also shown.
KW - Absorption spectra
KW - Fatty acids
KW - Non-destructive assay
KW - Photoadaptation
KW - Scattering correction
UR - http://www.scopus.com/inward/record.url?scp=67349245491&partnerID=8YFLogxK
U2 - 10.1007/s10811-008-9377-6
DO - 10.1007/s10811-008-9377-6
M3 - Article
AN - SCOPUS:67349245491
SN - 0921-8971
VL - 21
SP - 361
EP - 366
JO - Journal of Applied Phycology
JF - Journal of Applied Phycology
IS - 3
ER -