TY - JOUR
T1 - Ca2+ binding sites of the ryanodine receptor/Ca2+ release channel of sarcoplasmic reticulum
T2 - Low affinity binding site(S) as probed by terbium fluorescence
AU - Hadad, Nurit
AU - Zable, Anthony C.
AU - Abramson, Jonathan J.
AU - Shoshan-Barmatz, Varda
PY - 1994/10/7
Y1 - 1994/10/7
N2 - Fluorescence spectroscopy has been used to study the interaction of Tb3+ (as a Ca2+ analog) with the purified ryanodine receptor (RyR)/Ca2+ release channel of skeletal muscle sarcoplasmic reticulum. Tb3+ replaces Ca2+ in both the high- and the low-affinity sites. Occupation of the low-affinity site (inhibitory), but not of the high-affinity Ca2+ binding site (activating), by Tb3+ results in a strong enhanced green fluorescence (at 543 nm) and in an inhibition of ryanodine binding. The Tb3+ concentrations required for half-maximal enhanced fluorescence and inhibition of ryanodine binding were: 22.5 ± 2.5 μM (n = 4) and 22.3 ± 3.1 μM (n = 2), respectively. Tb3+ appears to bind to the protein at two or more cooperative sites (nH = 2.4) and to dissociate from these sites with three different rate constants (K-1.1 = 361 ± 250 min-1 (n = 6); K-1,2 = 0.45 ± 0.22 min-1 (n = 11); K-1,3 = 0.011 ± 0.013 min-1 (n = 7). The enhancement in Tb3+ fluorescence is very fast (K1 » 5 × 105 M-1), and it is quenched by EGTA, La3+, or Ca2+ addition. About 20% of the bound Tb3+ was not displaced by EGTA or Ca2+; suggesting its "occlusion" in the RyR. This is also reflected in the partially irreversible inhibition of ryanodine binding by Tb3+. Reconstitution of sarcoplasmic reticulum vesicles into a planar bilayer lipid membrane showed that the Ca2+ release channel was activated by submicromolar and inhibited by micromolar concentrations of Tb3+ and La3+. The Tb3+-activated channel showed an enhancement of the open dwell time of the channel. The results suggest that RyR/Ca2+ release channel undergoes conformational changes due to Tb3+ binding to the low-affinity Ca2+ binding site, and this binding results in the closing of the Ca2+ release channel.
AB - Fluorescence spectroscopy has been used to study the interaction of Tb3+ (as a Ca2+ analog) with the purified ryanodine receptor (RyR)/Ca2+ release channel of skeletal muscle sarcoplasmic reticulum. Tb3+ replaces Ca2+ in both the high- and the low-affinity sites. Occupation of the low-affinity site (inhibitory), but not of the high-affinity Ca2+ binding site (activating), by Tb3+ results in a strong enhanced green fluorescence (at 543 nm) and in an inhibition of ryanodine binding. The Tb3+ concentrations required for half-maximal enhanced fluorescence and inhibition of ryanodine binding were: 22.5 ± 2.5 μM (n = 4) and 22.3 ± 3.1 μM (n = 2), respectively. Tb3+ appears to bind to the protein at two or more cooperative sites (nH = 2.4) and to dissociate from these sites with three different rate constants (K-1.1 = 361 ± 250 min-1 (n = 6); K-1,2 = 0.45 ± 0.22 min-1 (n = 11); K-1,3 = 0.011 ± 0.013 min-1 (n = 7). The enhancement in Tb3+ fluorescence is very fast (K1 » 5 × 105 M-1), and it is quenched by EGTA, La3+, or Ca2+ addition. About 20% of the bound Tb3+ was not displaced by EGTA or Ca2+; suggesting its "occlusion" in the RyR. This is also reflected in the partially irreversible inhibition of ryanodine binding by Tb3+. Reconstitution of sarcoplasmic reticulum vesicles into a planar bilayer lipid membrane showed that the Ca2+ release channel was activated by submicromolar and inhibited by micromolar concentrations of Tb3+ and La3+. The Tb3+-activated channel showed an enhancement of the open dwell time of the channel. The results suggest that RyR/Ca2+ release channel undergoes conformational changes due to Tb3+ binding to the low-affinity Ca2+ binding site, and this binding results in the closing of the Ca2+ release channel.
UR - http://www.scopus.com/inward/record.url?scp=0028170670&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:0028170670
SN - 0021-9258
VL - 269
SP - 24864
EP - 24869
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
IS - 40
ER -