Ca2+ binding sites of the ryanodine receptor/Ca2+ release channel of sarcoplasmic reticulum. Low affinity binding site(s) as probed by terbium fluorescence

N. Hadad, A. C. Zable, J. J. Abramson, V. Shoshan-Barmatz

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20 Scopus citations

Abstract

Fluorescence spectroscopy has been used to study the interaction of Tb3+ (as a Ca2+ analog) with the purified ryanodine receptor (RyR)/Ca2+ release channel of skeletal muscle sarcoplasmic reticulum. Tb3+ replaces Ca2+ in both the high- and the low-affinity sites. Occupation of the low- affinity site (inhibitory), but not of the high-affinity Ca2+ binding site (activating), by Tb3+ results in a strong enhanced green fluorescence (at 543 nm) and in an inhibition of ryanodine binding. The Tb3+ concentrations required for half-maximal enhanced fluorescence and inhibition of ryanodine binding were: 22.5 ± 2.5 μM (n = 4) and 22.3 ± 3.1 μM (n = 2), respectively. Tb3+ appears to bind to the protein at two or more cooperative sites (n(H) = 2.4) and to dissociate from these sites with three different rate constants (K-1,1 = 361 ± 250 min-1 (n = 6); K-1,2 = 0.45 ± 0.22 min-1 (n = 11); K-1,3 = 0.011 ± 0.013 min-1 (n = 7). The enhancement in Tb3+ fluorescence is very fast (K1 >> 5 x 105 M-1 · min-1), and it is quenched by EGTA, La3+, or Ca2+ addition. About 20% of the bound Tb3+ was not displaced by EGTA or Ca2+; suggesting its 'occlusion' in the RyR. This is also reflected in the partially irreversible inhibition of ryanodine binding by Tb3+. Reconstitution of sarcoplasmic reticulum vesicles into a planar bilayer lipid membrane showed that the Ca2+ release channel was activated by submicromolar and inhibited by micromolar concentrations of Tb3+ and La3+. The Tb3+-activated channel showed an enhancement of the open dwell time of the channel. The results suggest that RyR/Ca2+ release channel undergoes conformational changes due to Tb3+ binding to the low-affinity Ca2+ binding site, and this binding results in the closing of the Ca2+ release channel.

Original languageEnglish
Pages (from-to)24864-24869
Number of pages6
JournalJournal of Biological Chemistry
Volume269
Issue number40
StatePublished - 1 Jan 1994

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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