Cell free DNA detected by a novel method in acute ST-elevation myocardial infarction patients

Avi Shimony, Doron Zahger, Harel Gilutz, Hagit Goldstein, Gennady Orlov, Miri Merkin, Aryeh Shalev, Reuben Ilia, Amos Douvdevani

Research output: Contribution to journalArticlepeer-review

57 Scopus citations

Abstract

Background: High levels of circulating cell free DNA (CFD) have been associated with poor prognosis in various diseases. Data pertaining to CFD in acute myocardial infarction (MI) are scarce. The available data have been obtained by either electrophoresis or polymerase chain reaction. We evaluated a novel method for the detection of CFD in patients with ST elevation myocardial infarction (STEMI) and examined its correlation with established markers of necrosis and ventricular function. Methods: Serum concentrations of CFD, troponin-T and creatine kinase (CK) were measured simultaneously in 16 randomly selected acute STEMI patients upon admission and at three more time points. 47 healthy subjects served as a control group. CFD was quantified by a novel rapid fluorometric assay. Ejection fraction (EF) was assessed by echocardiography. Results: Peak CFD levels were significantly higher in patients compared with controls (P 0.001) and correlated with peak levels of CK and troponin-T (R 0.79, P <0.001); R 0.65, P 0.006, respectively). Peak CFD levels tended to be associated with lower EF (P 0.075). Conclusion: With this method, CFD levels correlated with the levels of established markers of myocardial necrosis but not with EF. The kinetic pattern of CFD release after STEMI and its prognostic value require further investigation.

Original languageEnglish
Pages (from-to)109-111
Number of pages3
JournalAcute Cardiac Care
Volume12
Issue number3
DOIs
StatePublished - 1 Jan 2010

Keywords

  • Acute myocardial infarction
  • Cell free DNA
  • Marker

ASJC Scopus subject areas

  • Emergency Medicine
  • Radiology Nuclear Medicine and imaging
  • Cardiology and Cardiovascular Medicine

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