Cell proliferation in response to GABA in postnatal hippocampal slice culture

G. Ben-Yaakov; H. Golan

doi:10.1016/S0736-5748(03)00027-3

Cell proliferation in response to GABA in postnatal hippocampal slice culture

G. Ben-Yaakov, H. Golan

Recanati School for Community Health Professions

Research output: Contribution to journal › Article › peer-review

19 Scopus citations

Abstract

Recent studies have implicated the inhibitory neurotransmitter GABA in the regulation of cell migration and proliferation in the embryonic brain. Herein, we examine the possibility that these effects are maintained postnatally. Using 5 days postnatal hippocampus, we tested GABA effects on growth into an incision made in CA1 and the possibility that this response to GABA is mediated via GABA_A-receptor. Our data shows that GABA promotes neurite growth, cell proliferation and migration up to day 6 in vitro. GABA_A-receptor is not involved in the trophic response to either exogenous or endogenous GABA. Moreover, GABA induced a 20% increase in NGF secretion to the growth medium, in a similar time frame to its effect on growth. Elevated NGF secretion was suppressed by the inhibition of cell proliferation. These results suggest that GABA can promote growth in postnatal hippocampal tissue. The effect involves cell proliferation and NGF secretion and does not depend on GABA_A-receptor activation.

Original language	English
Pages (from-to)	153-157
Number of pages	5
Journal	International Journal of Developmental Neuroscience
Volume	21
Issue number	3
DOIs	https://doi.org/10.1016/S0736-5748(03)00027-3
State	Published - 1 Jan 2003

Keywords

Cell proliferation
GABA
Hippocampus
Organotypic slice culture

ASJC Scopus subject areas

Developmental Neuroscience
Developmental Biology

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10.1016/S0736-5748(03)00027-3

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title = "Cell proliferation in response to GABA in postnatal hippocampal slice culture",

abstract = "Recent studies have implicated the inhibitory neurotransmitter GABA in the regulation of cell migration and proliferation in the embryonic brain. Herein, we examine the possibility that these effects are maintained postnatally. Using 5 days postnatal hippocampus, we tested GABA effects on growth into an incision made in CA1 and the possibility that this response to GABA is mediated via GABAA-receptor. Our data shows that GABA promotes neurite growth, cell proliferation and migration up to day 6 in vitro. GABAA-receptor is not involved in the trophic response to either exogenous or endogenous GABA. Moreover, GABA induced a 20% increase in NGF secretion to the growth medium, in a similar time frame to its effect on growth. Elevated NGF secretion was suppressed by the inhibition of cell proliferation. These results suggest that GABA can promote growth in postnatal hippocampal tissue. The effect involves cell proliferation and NGF secretion and does not depend on GABAA-receptor activation.",

keywords = "Cell proliferation, GABA, Hippocampus, Organotypic slice culture",

author = "G. Ben-Yaakov and H. Golan",

note = "Funding Information: Supported by The Israeli Health Department grant to Dr. H. Golan. This study was performed in the framework of “research experience” which is part of Mr. Ben-Yaakov{\textquoteright}s requirements towards M.D. degree. ",

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AU - Golan, H.

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N2 - Recent studies have implicated the inhibitory neurotransmitter GABA in the regulation of cell migration and proliferation in the embryonic brain. Herein, we examine the possibility that these effects are maintained postnatally. Using 5 days postnatal hippocampus, we tested GABA effects on growth into an incision made in CA1 and the possibility that this response to GABA is mediated via GABAA-receptor. Our data shows that GABA promotes neurite growth, cell proliferation and migration up to day 6 in vitro. GABAA-receptor is not involved in the trophic response to either exogenous or endogenous GABA. Moreover, GABA induced a 20% increase in NGF secretion to the growth medium, in a similar time frame to its effect on growth. Elevated NGF secretion was suppressed by the inhibition of cell proliferation. These results suggest that GABA can promote growth in postnatal hippocampal tissue. The effect involves cell proliferation and NGF secretion and does not depend on GABAA-receptor activation.

AB - Recent studies have implicated the inhibitory neurotransmitter GABA in the regulation of cell migration and proliferation in the embryonic brain. Herein, we examine the possibility that these effects are maintained postnatally. Using 5 days postnatal hippocampus, we tested GABA effects on growth into an incision made in CA1 and the possibility that this response to GABA is mediated via GABAA-receptor. Our data shows that GABA promotes neurite growth, cell proliferation and migration up to day 6 in vitro. GABAA-receptor is not involved in the trophic response to either exogenous or endogenous GABA. Moreover, GABA induced a 20% increase in NGF secretion to the growth medium, in a similar time frame to its effect on growth. Elevated NGF secretion was suppressed by the inhibition of cell proliferation. These results suggest that GABA can promote growth in postnatal hippocampal tissue. The effect involves cell proliferation and NGF secretion and does not depend on GABAA-receptor activation.

KW - Cell proliferation

KW - GABA

KW - Hippocampus

KW - Organotypic slice culture

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JO - International Journal of Developmental Neuroscience

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ER -

Cell proliferation in response to GABA in postnatal hippocampal slice culture

Abstract

Keywords

ASJC Scopus subject areas

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