Cell rolling cytometer for characterizing dynamic adhesion of mesenchymal stem cells

Sungyoung Choi, Oren Levy, Mónica B. Coelho, Jeffrey M. Karp, Rohit Karnik

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

This paper reports a cell rolling cytometer (CRC) for controllably contacting and transporting cells in suspension using a three-dimensional microtopography coated with adhesion molecules, which enables quantification of cell-surface adhesion dynamics via transit time and lateral position at the single cell level. The cell rolling cytometer comprises slant ridges which not only enhance cell-surface interactions for cell tethering, but also direct the rolling of target cells. This approach enables simple, rapid quantification of cell adhesion to surface-bound molecules such as selectins at the single cell level.

Original languageEnglish
Title of host publicationProceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
PublisherChemical and Biological Microsystems Society
Pages431-433
Number of pages3
ISBN (Print)9780979806452
StatePublished - 1 Jan 2012
Externally publishedYes
Event16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 - Okinawa, Japan
Duration: 28 Oct 20121 Nov 2012

Publication series

NameProceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012

Conference

Conference16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
Country/TerritoryJapan
CityOkinawa
Period28/10/121/11/12

Keywords

  • Adhesion molecules
  • Cell rolling cytometer
  • Mesenchymal stem cell
  • Microfluidics

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering

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