Cellular imaging and folate receptor targeting delivery of gum kondagogu capped gold nanoparticles in cancer cells

Sathish Sundar Dhilip Kumar, Ayyavu Mahesh, M. Gover Antoniraj, Hanumant Singh Rathore, N. N. Houreld, Ruckmani Kandasamy

Research output: Contribution to journalArticlepeer-review

36 Scopus citations

Abstract

In this study, the green synthesis of gum kondagogu capped gold nanoparticles (GK-GNPs) was prepared using a naturally available polysaccharide. The anionic gum capped GK-GNPs enabled the successful coupling of folic acid (FA) and fluorescein isothiocyanate (FITC) to produce a fluorescently labelled GNP (F2-GNP). F2-GNPs were further characterized using different physicochemical methods Cellular viability, cellular imaging, and targeted delivery of F2-GNPs were further evaluated in both folate receptor positive (MCF-7) and folate receptor negative (A549) cancer cells. Physicochemical characterization revealed a nanoparticle with a small size (37 nm), smooth surface (surface charge of −23.7 mV), crystallinity of gold nanoparticles and existence of gum kondagogu in the F2-GNPs. Cellular uptake of F2-GNPs indicated a greater affinity towards folate receptor positive cells. This study shows that the F2-GNPs is as an effective nanocarrier for targeted drug delivery and cellular imaging via folate receptors.

Original languageEnglish
Pages (from-to)220-230
Number of pages11
JournalInternational Journal of Biological Macromolecules
Volume109
DOIs
StatePublished - 1 Apr 2018
Externally publishedYes

Keywords

  • Cancer cells
  • Cellular imaging
  • Folic acid
  • Gold nanoparticles
  • Polysaccharides

ASJC Scopus subject areas

  • Structural Biology
  • Biochemistry
  • Molecular Biology

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