Cellular spectroscopy and multiangle light scattering by flow cytometry: Optical test bench as a developmental tool

Gordon Wiegand, Itzhak Avital

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

We designed and fabricated a flow cytometry work bench for the purpose of testing optical schemes utilized in two specialized flow cytometry techniques. Fluorescent spectroscopy and multi-angle scattering of laser light has potential for generating novel classes of cell population information. The acquisition of full fluorescence spectrum of dye bound to living cells may associate specific function to spectral shift. Similarly, detection of 2 discrete angles of light scatter was shown to describe internal structures of cells based on refractive and diffractive properties. We utilized a flow cytometry electro- cells based on refractive and diffractive properties. We utilized a flow cytometry electro- optical test bench to develop and describe alternate light collection schemes for these two techniques. Mouse bone marrow was chosen as the subject of analysis because it contains a consistent mix of cells that possess distinct biophysical properties. More importantly, these cells are the source of various biological systems that are targeted by environmental stress and clinical disease.

Original languageEnglish
Title of host publicationImaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VIII
DOIs
StatePublished - 10 May 2010
Externally publishedYes
EventImaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VIII - San Francisco, CA, United States
Duration: 23 Jan 201025 Jan 2010

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume7568
ISSN (Print)1605-7422

Conference

ConferenceImaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VIII
Country/TerritoryUnited States
CitySan Francisco, CA
Period23/01/1025/01/10

Keywords

  • Analytical cytometry
  • Cellular spectroscopy
  • Multi-angle light scatter
  • Scanning monochrometer

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