Ceramide 1-phosphate (C1P) promotes cell migration. Involvement of a specific C1P receptor

María H. Granado; Patricia Gangoiti; Alberto Ouro; Lide Arana; Monika González; Miguel Trueba; Antonio Gómez-Muñoz

doi:10.1016/j.cellsig.2008.11.003

Ceramide 1-phosphate (C1P) promotes cell migration. Involvement of a specific C1P receptor

María H. Granado, Patricia Gangoiti, Alberto Ouro, Lide Arana, Monika González, Miguel Trueba, Antonio Gómez-Muñoz

Research output: Contribution to journal › Article › peer-review

127 Scopus citations

Abstract

Ceramide 1-phosphate (C1P) is a bioactive sphingolipid that is implicated in the regulation of cell homeostasis and the control of inflammation. It is mitogenic for fibroblasts and macrophages, and has been described as potent inhibitor of apoptosis. Using RAW 264.7 macrophages we have now discovered a new biological activity of C1P: stimulation of cell migration. This novel action can only be observed when C1P is applied exogenously to the cells in culture, and not by increasing the intracellular levels of C1P. This fact led to identify a specific receptor through which C1P stimulates cell migration. The receptor is coupled to G_i proteins and causes phosphorylation of extracellularly regulated kinases 1 and 2, and protein kinase B (also known as Akt) upon ligation with C1P. Inhibition of either of these pathways completely abolished C1P-stimulated macrophage migration. In addition, C1P stimulated the DNA binding activity of nuclear factor kappa B, and blockade of this transcription factor resulted in complete inhibition of macrophage migration. This newly identified receptor could be an important drug target for treatment of illnesses that are associated to inflammatory processes, or to diseases in which cell migration is a major cause of pathology, as it occurs in metastatic tumors.

Original language	English
Pages (from-to)	405-412
Number of pages	8
Journal	Cellular Signalling
Volume	21
Issue number	3
DOIs	https://doi.org/10.1016/j.cellsig.2008.11.003
State	Published - 1 Mar 2009
Externally published	Yes

Keywords

Ceramide
Macrophages
Mitogen-activated protein kinases
Phosphatidylinositol 3-kinase
Protein kinase B
Sphingolipids

ASJC Scopus subject areas

Cell Biology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1016/j.cellsig.2008.11.003

Cite this

@article{59828f71a90f40609b86cf4d4e801748,

title = "Ceramide 1-phosphate (C1P) promotes cell migration. Involvement of a specific C1P receptor",

abstract = "Ceramide 1-phosphate (C1P) is a bioactive sphingolipid that is implicated in the regulation of cell homeostasis and the control of inflammation. It is mitogenic for fibroblasts and macrophages, and has been described as potent inhibitor of apoptosis. Using RAW 264.7 macrophages we have now discovered a new biological activity of C1P: stimulation of cell migration. This novel action can only be observed when C1P is applied exogenously to the cells in culture, and not by increasing the intracellular levels of C1P. This fact led to identify a specific receptor through which C1P stimulates cell migration. The receptor is coupled to Gi proteins and causes phosphorylation of extracellularly regulated kinases 1 and 2, and protein kinase B (also known as Akt) upon ligation with C1P. Inhibition of either of these pathways completely abolished C1P-stimulated macrophage migration. In addition, C1P stimulated the DNA binding activity of nuclear factor kappa B, and blockade of this transcription factor resulted in complete inhibition of macrophage migration. This newly identified receptor could be an important drug target for treatment of illnesses that are associated to inflammatory processes, or to diseases in which cell migration is a major cause of pathology, as it occurs in metastatic tumors.",

keywords = "Ceramide, Macrophages, Mitogen-activated protein kinases, Phosphatidylinositol 3-kinase, Protein kinase B, Sphingolipids",

author = "Granado, {Mar{\'i}a H.} and Patricia Gangoiti and Alberto Ouro and Lide Arana and Monika Gonz{\'a}lez and Miguel Trueba and Antonio G{\'o}mez-Mu{\~n}oz",

year = "2009",

month = mar,

day = "1",

doi = "10.1016/j.cellsig.2008.11.003",

language = "English",

volume = "21",

pages = "405--412",

journal = "Cellular Signalling",

issn = "0898-6568",

publisher = "Elsevier Inc.",

number = "3",

}

TY - JOUR

T1 - Ceramide 1-phosphate (C1P) promotes cell migration. Involvement of a specific C1P receptor

AU - Granado, María H.

AU - Gangoiti, Patricia

AU - Ouro, Alberto

AU - Arana, Lide

AU - González, Monika

AU - Trueba, Miguel

AU - Gómez-Muñoz, Antonio

PY - 2009/3/1

Y1 - 2009/3/1

N2 - Ceramide 1-phosphate (C1P) is a bioactive sphingolipid that is implicated in the regulation of cell homeostasis and the control of inflammation. It is mitogenic for fibroblasts and macrophages, and has been described as potent inhibitor of apoptosis. Using RAW 264.7 macrophages we have now discovered a new biological activity of C1P: stimulation of cell migration. This novel action can only be observed when C1P is applied exogenously to the cells in culture, and not by increasing the intracellular levels of C1P. This fact led to identify a specific receptor through which C1P stimulates cell migration. The receptor is coupled to Gi proteins and causes phosphorylation of extracellularly regulated kinases 1 and 2, and protein kinase B (also known as Akt) upon ligation with C1P. Inhibition of either of these pathways completely abolished C1P-stimulated macrophage migration. In addition, C1P stimulated the DNA binding activity of nuclear factor kappa B, and blockade of this transcription factor resulted in complete inhibition of macrophage migration. This newly identified receptor could be an important drug target for treatment of illnesses that are associated to inflammatory processes, or to diseases in which cell migration is a major cause of pathology, as it occurs in metastatic tumors.

AB - Ceramide 1-phosphate (C1P) is a bioactive sphingolipid that is implicated in the regulation of cell homeostasis and the control of inflammation. It is mitogenic for fibroblasts and macrophages, and has been described as potent inhibitor of apoptosis. Using RAW 264.7 macrophages we have now discovered a new biological activity of C1P: stimulation of cell migration. This novel action can only be observed when C1P is applied exogenously to the cells in culture, and not by increasing the intracellular levels of C1P. This fact led to identify a specific receptor through which C1P stimulates cell migration. The receptor is coupled to Gi proteins and causes phosphorylation of extracellularly regulated kinases 1 and 2, and protein kinase B (also known as Akt) upon ligation with C1P. Inhibition of either of these pathways completely abolished C1P-stimulated macrophage migration. In addition, C1P stimulated the DNA binding activity of nuclear factor kappa B, and blockade of this transcription factor resulted in complete inhibition of macrophage migration. This newly identified receptor could be an important drug target for treatment of illnesses that are associated to inflammatory processes, or to diseases in which cell migration is a major cause of pathology, as it occurs in metastatic tumors.

KW - Ceramide

KW - Macrophages

KW - Mitogen-activated protein kinases

KW - Phosphatidylinositol 3-kinase

KW - Protein kinase B

KW - Sphingolipids

UR - http://www.scopus.com/inward/record.url?scp=58249129925&partnerID=8YFLogxK

U2 - 10.1016/j.cellsig.2008.11.003

DO - 10.1016/j.cellsig.2008.11.003

M3 - Article

C2 - 19041940

AN - SCOPUS:58249129925

SN - 0898-6568

VL - 21

SP - 405

EP - 412

JO - Cellular Signalling

JF - Cellular Signalling

IS - 3

ER -

Ceramide 1-phosphate (C1P) promotes cell migration. Involvement of a specific C1P receptor

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this