Channel modulators affect PGE2 binding to bovine aortic endothelial cells

Gilad Rimon, Mazal Rubin

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

PGE2, PGF and the thromboxane agonist U-46619 bind to bovine aortic endothelial cells and compete on the same binding site with similar affinity. In addition, binding remains unaffected by prolonged exposure to the ligand. These characteristics differ significantly from those of any known G-coupled prostaglandin receptor. Binding of PGE2 to the cells is reduced in the presence of the cyclic nucleotides cGMP and cAMP, and is unaffected by protein kinase inhibitors. Removal of permeable cyclic nucleotides from the cell medium results in a fast and complete restoration of PGE2 binding to the cells, suggesting that both cyclic nucleotides reduce PGE2 binding by a reversible interaction with the prostaglandin-binding site, without the involvement of second messenger-activated protein kinases. Our data further show that binding of prostaglandins to bovine aortic endothelial cells is sensitive to heavy metals and to activators and blockers of calcium, ATP-sensitive K(+) and chloride channels. Nickel, a specific cyclic nucleotide-gated (CNG) channel activator, decreases PGE2 binding and so do the CNG channel activators Rp-8-Br-PET-cGMPS and Sp-8-Br-PET-cGMPS. On the other hand, the calcium channel blockers pimozide, diltiazem as well as LY-83,583, a guanylate cyclase inhibitor, which were reported to block CNG channels, enhance PGE2 binding. The sensitivity of PGE2 binding to selective CNG channel modifying agents, as well as the rapid and reversible interaction with cyclic nucleotides, may suggest that the common low-affinity prostanoid-binding site on bovine aortic endothelial cells is associated with a molecular entity, which possess several properties of a CNG channel.

Original languageEnglish
Pages (from-to)113-120
Number of pages8
JournalBiochimica et Biophysica Acta - General Subjects
Volume1570
Issue number2
DOIs
StatePublished - 15 Mar 2002

Keywords

  • Bovine
  • CNG channel
  • Channel modulator
  • Endothelial cell
  • PGE

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