Characterization of acetohydroxy acid synthase activity in the archaeon Haloferax volcanii

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Abstract

Whereas the biochemistry of acetohydroxy acid synthase has been extensively studied in bacteria and eukaryotes, relatively little is known about the enzyme in archaea, the third kingdom of life. The present study biochemically characterizes acetohydroxy acid synthase activity in the halophilic archaea Haloferax volcanii. In addressing ion requirements, enzyme inhibition and antibody labeling, the results reveal that, except for its elevated salt requirements, the haloarchaeal enzyme is remarkably similar to its bacterial counterpart. Copyright (C) 2000 Elsevier Science Inc.

Original languageEnglish
Pages (from-to)205-210
Number of pages6
JournalComparative Biochemistry and Physiology - Part B: Biochemistry and Molecular Biology
Volume125
Issue number2
DOIs
StatePublished - 1 Jan 2000

Keywords

  • Acetohydroxy acid synthase
  • Amino acid biosynthesis
  • Archaea
  • Branched-chain amino acids
  • Euryarchaeota
  • Haloferax volcanii
  • Halophile

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