Whereas the biochemistry of acetohydroxy acid synthase has been extensively studied in bacteria and eukaryotes, relatively little is known about the enzyme in archaea, the third kingdom of life. The present study biochemically characterizes acetohydroxy acid synthase activity in the halophilic archaea Haloferax volcanii. In addressing ion requirements, enzyme inhibition and antibody labeling, the results reveal that, except for its elevated salt requirements, the haloarchaeal enzyme is remarkably similar to its bacterial counterpart. Copyright (C) 2000 Elsevier Science Inc.
|Number of pages||6|
|Journal||Comparative Biochemistry and Physiology - Part B: Biochemistry and Molecular Biology|
|State||Published - 1 Jan 2000|
- Acetohydroxy acid synthase
- Amino acid biosynthesis
- Branched-chain amino acids
- Haloferax volcanii