Characterization of autochthonous bacteria capable for degradation of residual organic pollutants of pulp paper mill effluent by biostimulation process

Pooja Sharma, Sonam Tripathi, Preeti Chaturvedi, Ram Chandra

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4 Scopus citations

Abstract

The purpose of this study is remediation of residual organic pollutants from effluent by autochthonous bacterial community in biostimulation process. Discharged effluent showed high TDS (549 mg L-1), tss (59 mg L-1), COD (20349 mg L-1) and BOD (25946 mg L-1), value. The level of total phenol (421 mg L-1), nitrogen (156 mg L-1), sulphate (1854 mg L-1), phosphorus (176 mg L-1) chlorine (2.01 mg L-1), sodium (75 mg L-1) and potassium (8.4 mg L-1) along with various heavy metals (Fe, 75.23; Zn, 15.60; Cu, 4.1; Cr, 3.12; Cd, 0.324; Mn, 13.24; and Ni, 4.01 mg L-1) were noted above the permissible limit of Environmental Protection Act. The result revealed that the reduction of the physico-chemical parameter of pollutants were above 50% after biostimulation process, this confirmed the potentiality of growing autochthonous bacterial community responsible for bioremediation. The comparative UV-Vis spectroscopy showed reduction in the absorption spectra of degraded sample. Further, GC-MS analysis showed major organic pollutants i.e. Octadecanoic acid, Hexadecanoic acid, citral, benzoic acid, and 2, 6’-Di-hydroxy acetophenone, bis (trimethylsilyl) ether, were detected in control few compounds were degraded while there was formation of some new metabolic products also. Few pollutants persisted in the degraded sample as recalcitrant toxicant and causes environmental toxicity and hormonal imbalance as endocrine-disrupting chemicals (EDCs). But the detailed knowledge and characterization of organic pollutants are not available yet regarding their properties. The SEM image showed the diversity of bacterial community in biostimulation responsible for utilisation of various detected compounds. The growing bacterial communities were identified as potential bacterial strains as Aeromonas salmonicida, BBAUPS-1 (MN294457.1) and Bacillus sp. BBAUPS-2 (MN238724.1) responsible for the remediation of residual organic pollutants. Further, Evaluation of toxicity parameter of effluent by seed germination test of Triticum aestivum and Cicer arietinum inhibited the seed germination upto 80%. Hence, this study revealed that the biostimulation process is a good technique for detoxification and degradation effluent.

Original languageEnglish
Pages (from-to)1181-1194
Number of pages14
JournalJournal of Pure and Applied Microbiology
Volume14
Issue number2
DOIs
StatePublished - 1 Jun 2020
Externally publishedYes

Keywords

  • Effluent detoxification
  • Endocrine-disrupting chemicals
  • Environmental toxicity
  • Organic pollutants
  • Phytotoxicity

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