Characterization of DIDS-sensitive ATP accumulation in brain synaptic vesicles

Ran Zalk; Varda Shoshan-Barmatz

doi:10.1016/j.febslet.2006.09.055

Characterization of DIDS-sensitive ATP accumulation in brain synaptic vesicles

Ran Zalk, Varda Shoshan-Barmatz

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

ATP is an excitatory neurotransmitter in the central and peripheral nervous system. We investigated ATP accumulation in highly purified brain synaptic vesicles (SVs). Based on the amount of ATP accumulated in SVs under the conditions used, ATP is not transported against a concentration gradient but rather appears to have a ΔμH⁺-independent mechanism. ATP transport was inhibited by DIDS and NEM, but was not affected by Mg²⁺ or by pre-incubation with nucleotides. These results suggest a unique transport mechanism that does not involve exchange with other nucleotides or protons, unlike other known neurotransmitter transport systems.

Original language	English
Pages (from-to)	5894-5898
Number of pages	5
Journal	FEBS Letters
Volume	580
Issue number	25
DOIs	https://doi.org/10.1016/j.febslet.2006.09.055
State	Published - 30 Oct 2006
Externally published	Yes

Keywords

ATP
DIDS
Synaptic vesicles
Transport

Access to Document

10.1016/j.febslet.2006.09.055

Cite this

@article{7ce771146a014a6687f6c9100439c462,

title = "Characterization of DIDS-sensitive ATP accumulation in brain synaptic vesicles",

abstract = "ATP is an excitatory neurotransmitter in the central and peripheral nervous system. We investigated ATP accumulation in highly purified brain synaptic vesicles (SVs). Based on the amount of ATP accumulated in SVs under the conditions used, ATP is not transported against a concentration gradient but rather appears to have a ΔμH+-independent mechanism. ATP transport was inhibited by DIDS and NEM, but was not affected by Mg2+ or by pre-incubation with nucleotides. These results suggest a unique transport mechanism that does not involve exchange with other nucleotides or protons, unlike other known neurotransmitter transport systems.",

keywords = "ATP, DIDS, Synaptic vesicles, Transport",

author = "Ran Zalk and Varda Shoshan-Barmatz",

year = "2006",

month = oct,

day = "30",

doi = "10.1016/j.febslet.2006.09.055",

language = "English",

volume = "580",

pages = "5894--5898",

journal = "FEBS Letters",

issn = "0014-5793",

publisher = "Wiley-Blackwell",

number = "25",

}

TY - JOUR

T1 - Characterization of DIDS-sensitive ATP accumulation in brain synaptic vesicles

AU - Zalk, Ran

AU - Shoshan-Barmatz, Varda

PY - 2006/10/30

Y1 - 2006/10/30

N2 - ATP is an excitatory neurotransmitter in the central and peripheral nervous system. We investigated ATP accumulation in highly purified brain synaptic vesicles (SVs). Based on the amount of ATP accumulated in SVs under the conditions used, ATP is not transported against a concentration gradient but rather appears to have a ΔμH+-independent mechanism. ATP transport was inhibited by DIDS and NEM, but was not affected by Mg2+ or by pre-incubation with nucleotides. These results suggest a unique transport mechanism that does not involve exchange with other nucleotides or protons, unlike other known neurotransmitter transport systems.

AB - ATP is an excitatory neurotransmitter in the central and peripheral nervous system. We investigated ATP accumulation in highly purified brain synaptic vesicles (SVs). Based on the amount of ATP accumulated in SVs under the conditions used, ATP is not transported against a concentration gradient but rather appears to have a ΔμH+-independent mechanism. ATP transport was inhibited by DIDS and NEM, but was not affected by Mg2+ or by pre-incubation with nucleotides. These results suggest a unique transport mechanism that does not involve exchange with other nucleotides or protons, unlike other known neurotransmitter transport systems.

KW - ATP

KW - DIDS

KW - Synaptic vesicles

KW - Transport

UR - http://www.scopus.com/inward/record.url?scp=33750042307&partnerID=8YFLogxK

U2 - 10.1016/j.febslet.2006.09.055

DO - 10.1016/j.febslet.2006.09.055

M3 - Article

AN - SCOPUS:33750042307

VL - 580

SP - 5894

EP - 5898

JO - FEBS Letters

JF - FEBS Letters

SN - 0014-5793

IS - 25

ER -

Characterization of DIDS-sensitive ATP accumulation in brain synaptic vesicles

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this