Characterization of intraperitoneal cytokines and nitrites in women with severe ovarian hyperstimulation syndrome

A. Revel, V. Barak, Y. Lavy, E. Anteby, Y. Abramov, J. J. Schenker, A. Amit, Z. Finci-Yeheskel, M. Mayer, A. Simon, N. Laufer, A. Hurwitz

Research output: Contribution to journalArticlepeer-review

70 Scopus citations


Objective: To assess the potential involvement of cytokines and nitrites in the hyperpermeability characterizing the ovarian hyperstimulation syndrome (OHSS). Design: A controlled clinical study comparing peritoneal fluid (PF) from patients with severe OHSS and from non-OHSS controls. Setting: Women hospitalized with severe OHSS in three tertiary medical centers. Patients: Twelve patients with severe OHSS necessitating paracentesis and 20 non-OHSS controls. Interventions: The criteria for ultrasound-guided paracentesis were tense ascites, hydrothorax, hemoconcentration, or oliguria. Main Outcome Measures: Interleukin (IL) 1β IL-1 receptor agonist, IL-2, IL-6, IL-8, and tumor necrosis factor a (TNFα) levels in PF were assayed by ELISA; nitrites were measured by the 'Griess' reaction. Estradiol and P were determined by RIA. Results: Ovarian hyperstimulation syndrome patients had significantly higher PF IL-6 (3,523 versus 30 pg/mL), TNFα (14 versus 4.2 pg/mL), and IL- 8 (1,695 versus 900 pg/mL). In the serum, only IL-6 levels were significantly higher (375 versus 11 pg/mL). Conversely, nitrite levels were significantly lower in PF of OHSS patients (0.5 versus 34 nmol/mL). Interleukin 1 levels were higher and IL-1 receptor antagonist levels were lower in OHSS patients, suggesting potentially increased biologic potency of IL-1. Conclusion: These findings suggest that these substances could be involved in mediating the capillary hyperpermeability characterizing this syndrome.

Original languageEnglish
Pages (from-to)66-71
Number of pages6
JournalFertility and Sterility
Issue number1
StatePublished - 1 Jan 1996
Externally publishedYes


  • OHSS
  • ascites
  • cytokines
  • nitric oxide

ASJC Scopus subject areas

  • Reproductive Medicine
  • Obstetrics and Gynecology


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