TY - JOUR
T1 - Characterization of murine A-raf, a new oncogene related to the v-raf oncogene.
AU - Huleihel, M.
AU - Goldsborough, M.
AU - Cleveland, J.
AU - Gunnell, M.
AU - Bonner, T.
AU - Rapp, U. R.
PY - 1986/1/1
Y1 - 1986/1/1
N2 - A 1.6-kilobase cDNA (A-raf) has been isolated from a murine spleen cDNA library which encodes part of a protein related to the raf oncogene. Its amino acid sequence has 85% homology to raf in a central portion of 100 amino acids. In contrast to raf, A-raf shows a highly restricted tissue distribution of expression, with highest levels observed in epididymis, followed by intestine. When incorporated into a retrovirus, the resulting gag-A-raf fusion gene causes transformation in vitro and induces tumors in newborn mice. Thus, A-raf represents a new proto-oncogene. Transformation by A-raf is independent of ras gene function, as is the case for raf and mos but not other oncogenes.
AB - A 1.6-kilobase cDNA (A-raf) has been isolated from a murine spleen cDNA library which encodes part of a protein related to the raf oncogene. Its amino acid sequence has 85% homology to raf in a central portion of 100 amino acids. In contrast to raf, A-raf shows a highly restricted tissue distribution of expression, with highest levels observed in epididymis, followed by intestine. When incorporated into a retrovirus, the resulting gag-A-raf fusion gene causes transformation in vitro and induces tumors in newborn mice. Thus, A-raf represents a new proto-oncogene. Transformation by A-raf is independent of ras gene function, as is the case for raf and mos but not other oncogenes.
UR - http://www.scopus.com/inward/record.url?scp=0022755881&partnerID=8YFLogxK
U2 - 10.1128/MCB.6.7.2655
DO - 10.1128/MCB.6.7.2655
M3 - Article
AN - SCOPUS:0022755881
SN - 0270-7306
VL - 6
SP - 2655
EP - 2662
JO - Molecular and Cellular Biology
JF - Molecular and Cellular Biology
IS - 7
ER -