Characterization of murine A-raf, a new oncogene related to the v-raf oncogene.

M. Huleihel, M. Goldsborough, J. Cleveland, M. Gunnell, T. Bonner, U. R. Rapp

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A 1.6-kilobase cDNA (A-raf) has been isolated from a murine spleen cDNA library which encodes part of a protein related to the raf oncogene. Its amino acid sequence has 85% homology to raf in a central portion of 100 amino acids. In contrast to raf, A-raf shows a highly restricted tissue distribution of expression, with highest levels observed in epididymis, followed by intestine. When incorporated into a retrovirus, the resulting gag-A-raf fusion gene causes transformation in vitro and induces tumors in newborn mice. Thus, A-raf represents a new proto-oncogene. Transformation by A-raf is independent of ras gene function, as is the case for raf and mos but not other oncogenes.

Original languageEnglish
Pages (from-to)2655-2662
Number of pages8
JournalMolecular and Cellular Biology
Issue number7
StatePublished - 1 Jan 1986


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