Characterization of mutants of the red microalga Porphyridium aerugineum (Rhodophyceae) resistant to DCMU and atrazine

Sigalit Boura-Halfon, Moshe Rise, Shoshana Arad, Alex Sivan

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9 Scopus citations

Abstract

In an attempt to obtain genetic markers in the red microalga Porphyridium aerugineum Geitler, two stable spontaneous mutants were isolated that were resistant to the herbicides diuron [3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU) (designated D9)] and atrazine [2-chloro-4-ethylamino-(isopropylamino)-1,3,5-triazine (designated A 10)]. The mutant resistant to DCMU was also found to be resistant to atrazine, whereas the amine-resistant mutant proved sensitive to DCMU. When the wild-type P. aerugineum was grown in the presence of the herbicides, the cellular contents of chlorophyll a, phycocyanin, zeaxanthin, and protein were higher, but the amounts of β-carotene and of cell wall polysaccharide were lower as compared to cells grown in herbicide-free medium. In the mutants, the herbicides did not have a significant effect on these cellular features.

Original languageEnglish
Pages (from-to)479-487
Number of pages9
JournalPhycologia
Volume36
Issue number6
DOIs
StatePublished - 1 Jan 1997

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