Characterization of oligonucleotide transport into living cells

S. L. Loke; C. A. Stein; X. H. Zhang; K. Mori; M. Nakanishi; C. Subasinghe; J. S. Cohen; L. M. Neckers

doi:10.1073/pnas.86.10.3474

Characterization of oligonucleotide transport into living cells

S. L. Loke, C. A. Stein, X. H. Zhang, K. Mori, M. Nakanishi, C. Subasinghe, J. S. Cohen, L. M. Neckers

Research output: Contribution to journal › Article › peer-review

774 Scopus citations

Abstract

Addition of antisense oligonucleotides to cell cultures has been used to specifically inhibit gene expression. We have investigated the mechanism by which oligonucleotides enter living cells. These compounds are taken up by cells in a saturable, size-dependent manner compatible with receptor-mediated endocytosis. Polynucleotides of any length are competitive inhibitors of oligomer transport, providing they possess a 5'-phosphate moiety. Using oligo(dT)-cellulose for affinity purification, we have identified an 80-kDa surface protein that may mediate transport. Knowledge of the oligonucleotide transport mechanism should facilitate the design of more effective synthetic antisense oligomers as potential clinical agents.

Original language	English
Pages (from-to)	3474-3478
Number of pages	5
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	86
Issue number	10
DOIs	https://doi.org/10.1073/pnas.86.10.3474
State	Published - 1 Jan 1989
Externally published	Yes

ASJC Scopus subject areas

General

Access to Document

10.1073/pnas.86.10.3474

Cite this

@article{1e685f550c4043c7965ad0a82d2a2e6b,

title = "Characterization of oligonucleotide transport into living cells",

abstract = "Addition of antisense oligonucleotides to cell cultures has been used to specifically inhibit gene expression. We have investigated the mechanism by which oligonucleotides enter living cells. These compounds are taken up by cells in a saturable, size-dependent manner compatible with receptor-mediated endocytosis. Polynucleotides of any length are competitive inhibitors of oligomer transport, providing they possess a 5'-phosphate moiety. Using oligo(dT)-cellulose for affinity purification, we have identified an 80-kDa surface protein that may mediate transport. Knowledge of the oligonucleotide transport mechanism should facilitate the design of more effective synthetic antisense oligomers as potential clinical agents.",

author = "Loke, {S. L.} and Stein, {C. A.} and Zhang, {X. H.} and K. Mori and M. Nakanishi and C. Subasinghe and Cohen, {J. S.} and Neckers, {L. M.}",

year = "1989",

month = jan,

day = "1",

doi = "10.1073/pnas.86.10.3474",

language = "English",

volume = "86",

pages = "3474--3478",

journal = "Proceedings of the National Academy of Sciences of the United States of America",

issn = "0027-8424",

publisher = "National Academy of Sciences",

number = "10",

}

TY - JOUR

T1 - Characterization of oligonucleotide transport into living cells

AU - Loke, S. L.

AU - Stein, C. A.

AU - Zhang, X. H.

AU - Mori, K.

AU - Nakanishi, M.

AU - Subasinghe, C.

AU - Cohen, J. S.

AU - Neckers, L. M.

PY - 1989/1/1

Y1 - 1989/1/1

N2 - Addition of antisense oligonucleotides to cell cultures has been used to specifically inhibit gene expression. We have investigated the mechanism by which oligonucleotides enter living cells. These compounds are taken up by cells in a saturable, size-dependent manner compatible with receptor-mediated endocytosis. Polynucleotides of any length are competitive inhibitors of oligomer transport, providing they possess a 5'-phosphate moiety. Using oligo(dT)-cellulose for affinity purification, we have identified an 80-kDa surface protein that may mediate transport. Knowledge of the oligonucleotide transport mechanism should facilitate the design of more effective synthetic antisense oligomers as potential clinical agents.

AB - Addition of antisense oligonucleotides to cell cultures has been used to specifically inhibit gene expression. We have investigated the mechanism by which oligonucleotides enter living cells. These compounds are taken up by cells in a saturable, size-dependent manner compatible with receptor-mediated endocytosis. Polynucleotides of any length are competitive inhibitors of oligomer transport, providing they possess a 5'-phosphate moiety. Using oligo(dT)-cellulose for affinity purification, we have identified an 80-kDa surface protein that may mediate transport. Knowledge of the oligonucleotide transport mechanism should facilitate the design of more effective synthetic antisense oligomers as potential clinical agents.

UR - http://www.scopus.com/inward/record.url?scp=0345570537&partnerID=8YFLogxK

U2 - 10.1073/pnas.86.10.3474

DO - 10.1073/pnas.86.10.3474

M3 - Article

C2 - 2726730

AN - SCOPUS:0345570537

SN - 0027-8424

VL - 86

SP - 3474

EP - 3478

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

IS - 10

ER -

Characterization of oligonucleotide transport into living cells

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this