Characterization of Plasma Membrane ATPase from Roots of Atriplex nummularia

David Mills, Thomas K. Hodges

    Research output: Contribution to journalArticlepeer-review

    11 Scopus citations

    Abstract

    Plasma membranes were isolated from «high salt» roots of Atriplex nummularia and Avena sativa by discontinuous sucrose gradients. Inhibition by vanadate and insensitivity to molybdate indicated that the 34/45 % sucrose interface was highly enriched in plasma membrane ATPase. The enzymes from the two species were similar in most respects: i.e. (1) ΔKCI-ATPase and ΔNaCl-ATPase kinetics over the range 1–200mM, (2) lack of synergistic response to Na+ and K+, (3) pH profiles of Mg-ATPase, ΔKCl-ATPase, and ΔKN03-ATPase activities, (4) Km and Vmax values of Mg-ATPase, (5) order of enhancement by cations. The Atriplex enzyme was less stimulated by Na+ and was more sensitive to anions than the oat enzyme. In oat, NaCl (50 mM) in the nutrient solution caused a 25 % and 40 % reduction of ΔNaCl-ATPase and ΔKCl-ATPase activity, respectively; in Atriplex, NaCl in the medium caused a slight increase in these activities. However, in view of the overall similarities between the two plant ATPases, the data suggest that this enzyme is essentially the same in the two species. The differences between the two species in Na+ transport in the roots are probably unrelated to the plasma membrane ATPase.

    Original languageEnglish
    Pages (from-to)513-519
    Number of pages7
    JournalJournal of Plant Physiology
    Volume132
    Issue number5
    DOIs
    StatePublished - 1 Jan 1988

    Keywords

    • A triplex nummularia
    • Avena sativa
    • DES
    • DES
    • EDAC
    • EDAC
    • NaCl
    • PNP
    • PNP
    • diethylstilbestrol
    • diethylstilbestrol
    • ethyl-3-(3-dimethyl-amino-propyl carbodiimide)
    • ethyl-3-(3-dimethyl-amino-propyl carbodiimide)
    • glycophyte
    • halophyte
    • p-nitrophenyl phosphate
    • p-nitrophenyl phosphate
    • plasma membrane ATPase
    • sucrose gradient

    ASJC Scopus subject areas

    • Physiology
    • Agronomy and Crop Science
    • Plant Science

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