Characterization of the transcriptome of chorioamniotic membranes at the site of rupture in spontaneous labor at term

Chia Ling Nhan-Chang; Roberto Romero; Adi L. Tarca; Pooja Mittal; Juan Pedro Kusanovic; Offer Erez; Shali Mazaki-Tovi; Tinnakorn Chaiworapongsa; John Hotra; Nandor Gabor Than; Jung Sun Kim; Sonia S. Hassan; Chong Jai Kim

doi:10.1016/j.ajog.2010.02.045

Characterization of the transcriptome of chorioamniotic membranes at the site of rupture in spontaneous labor at term

Chia Ling Nhan-Chang, Roberto Romero, Adi L. Tarca, Pooja Mittal, Juan Pedro Kusanovic, Offer Erez, Shali Mazaki-Tovi, Tinnakorn Chaiworapongsa, John Hotra, Nandor Gabor Than, Jung Sun Kim, Sonia S. Hassan, Chong Jai Kim

Research output: Contribution to journal › Article › peer-review

61 Scopus citations

Abstract

Objective: The purpose of this study was to compare the transcriptome between the site of membrane rupture and the chorioamniotic membranes away from the site of rupture. Study Design: The transcriptome of amnion and chorion (n = 20 each) from and distal to the site of rupture from women with spontaneous labor and vaginal delivery at term after spontaneous rupture of membranes was profiled with Illumina HumanHT-12 microarrays. Selected genes were validated with the use of quantitative reverse transcription-polymerase chain reaction. Results: Six hundred seventy-seven genes were differentially expressed in the chorion between the rupture and nonrupture sites (false discovery rate <0.1; fold change >1.5). Quantitative reverse transcription-polymerase chain reaction confirmed the differential expression in 10 of 14 genes. Enriched biological processes included anatomic structure development, cell adhesion and signal transduction. Extracellular matrix-receptor interaction was the most impacted signaling pathway. Conclusion: The transcriptome of fetal membranes after spontaneous rupture of membranes in term labor is characterized by region- and tissue-specific differential expression of genes that are involved in signature pathways, which include extracellular matrix-receptor interactions.

Original language	English
Pages (from-to)	462.e1-462.e41
Journal	American Journal of Obstetrics and Gynecology
Volume	202
Issue number	5
DOIs	https://doi.org/10.1016/j.ajog.2010.02.045
State	Published - 1 Jan 2010
Externally published	Yes

Keywords

chorion
extracellular matrix
inflammation
labor
microarray
parturition
spontaneous rupture of membranes

ASJC Scopus subject areas

Obstetrics and Gynecology

Access to Document

10.1016/j.ajog.2010.02.045

Cite this

Nhan-Chang, C. L., Romero, R., Tarca, A. L., Mittal, P., Kusanovic, J. P., Erez, O., Mazaki-Tovi, S., Chaiworapongsa, T., Hotra, J., Than, N. G., Kim, J. S., Hassan, S. S., & Kim, C. J. (2010). Characterization of the transcriptome of chorioamniotic membranes at the site of rupture in spontaneous labor at term. American Journal of Obstetrics and Gynecology, 202(5), 462.e1-462.e41. https://doi.org/10.1016/j.ajog.2010.02.045

@article{8d6d9bb068d4401e9a78644627e00534,

title = "Characterization of the transcriptome of chorioamniotic membranes at the site of rupture in spontaneous labor at term",

abstract = "Objective: The purpose of this study was to compare the transcriptome between the site of membrane rupture and the chorioamniotic membranes away from the site of rupture. Study Design: The transcriptome of amnion and chorion (n = 20 each) from and distal to the site of rupture from women with spontaneous labor and vaginal delivery at term after spontaneous rupture of membranes was profiled with Illumina HumanHT-12 microarrays. Selected genes were validated with the use of quantitative reverse transcription-polymerase chain reaction. Results: Six hundred seventy-seven genes were differentially expressed in the chorion between the rupture and nonrupture sites (false discovery rate <0.1; fold change >1.5). Quantitative reverse transcription-polymerase chain reaction confirmed the differential expression in 10 of 14 genes. Enriched biological processes included anatomic structure development, cell adhesion and signal transduction. Extracellular matrix-receptor interaction was the most impacted signaling pathway. Conclusion: The transcriptome of fetal membranes after spontaneous rupture of membranes in term labor is characterized by region- and tissue-specific differential expression of genes that are involved in signature pathways, which include extracellular matrix-receptor interactions.",

keywords = "chorion, extracellular matrix, inflammation, labor, microarray, parturition, spontaneous rupture of membranes",

author = "Nhan-Chang, {Chia Ling} and Roberto Romero and Tarca, {Adi L.} and Pooja Mittal and Kusanovic, {Juan Pedro} and Offer Erez and Shali Mazaki-Tovi and Tinnakorn Chaiworapongsa and John Hotra and Than, {Nandor Gabor} and Kim, {Jung Sun} and Hassan, {Sonia S.} and Kim, {Chong Jai}",

year = "2010",

month = jan,

day = "1",

doi = "10.1016/j.ajog.2010.02.045",

language = "English",

volume = "202",

pages = "462.e1--462.e41",

journal = "American Journal of Obstetrics and Gynecology",

issn = "0002-9378",

publisher = "Elsevier Inc.",

number = "5",

}

Nhan-Chang, CL, Romero, R, Tarca, AL, Mittal, P, Kusanovic, JP, Erez, O, Mazaki-Tovi, S, Chaiworapongsa, T, Hotra, J, Than, NG, Kim, JS, Hassan, SS & Kim, CJ 2010, 'Characterization of the transcriptome of chorioamniotic membranes at the site of rupture in spontaneous labor at term', American Journal of Obstetrics and Gynecology, vol. 202, no. 5, pp. 462.e1-462.e41. https://doi.org/10.1016/j.ajog.2010.02.045

TY - JOUR

T1 - Characterization of the transcriptome of chorioamniotic membranes at the site of rupture in spontaneous labor at term

AU - Nhan-Chang, Chia Ling

AU - Romero, Roberto

AU - Tarca, Adi L.

AU - Mittal, Pooja

AU - Kusanovic, Juan Pedro

AU - Erez, Offer

AU - Mazaki-Tovi, Shali

AU - Chaiworapongsa, Tinnakorn

AU - Hotra, John

AU - Than, Nandor Gabor

AU - Kim, Jung Sun

AU - Hassan, Sonia S.

AU - Kim, Chong Jai

PY - 2010/1/1

Y1 - 2010/1/1

N2 - Objective: The purpose of this study was to compare the transcriptome between the site of membrane rupture and the chorioamniotic membranes away from the site of rupture. Study Design: The transcriptome of amnion and chorion (n = 20 each) from and distal to the site of rupture from women with spontaneous labor and vaginal delivery at term after spontaneous rupture of membranes was profiled with Illumina HumanHT-12 microarrays. Selected genes were validated with the use of quantitative reverse transcription-polymerase chain reaction. Results: Six hundred seventy-seven genes were differentially expressed in the chorion between the rupture and nonrupture sites (false discovery rate <0.1; fold change >1.5). Quantitative reverse transcription-polymerase chain reaction confirmed the differential expression in 10 of 14 genes. Enriched biological processes included anatomic structure development, cell adhesion and signal transduction. Extracellular matrix-receptor interaction was the most impacted signaling pathway. Conclusion: The transcriptome of fetal membranes after spontaneous rupture of membranes in term labor is characterized by region- and tissue-specific differential expression of genes that are involved in signature pathways, which include extracellular matrix-receptor interactions.

AB - Objective: The purpose of this study was to compare the transcriptome between the site of membrane rupture and the chorioamniotic membranes away from the site of rupture. Study Design: The transcriptome of amnion and chorion (n = 20 each) from and distal to the site of rupture from women with spontaneous labor and vaginal delivery at term after spontaneous rupture of membranes was profiled with Illumina HumanHT-12 microarrays. Selected genes were validated with the use of quantitative reverse transcription-polymerase chain reaction. Results: Six hundred seventy-seven genes were differentially expressed in the chorion between the rupture and nonrupture sites (false discovery rate <0.1; fold change >1.5). Quantitative reverse transcription-polymerase chain reaction confirmed the differential expression in 10 of 14 genes. Enriched biological processes included anatomic structure development, cell adhesion and signal transduction. Extracellular matrix-receptor interaction was the most impacted signaling pathway. Conclusion: The transcriptome of fetal membranes after spontaneous rupture of membranes in term labor is characterized by region- and tissue-specific differential expression of genes that are involved in signature pathways, which include extracellular matrix-receptor interactions.

KW - chorion

KW - extracellular matrix

KW - inflammation

KW - labor

KW - microarray

KW - parturition

KW - spontaneous rupture of membranes

UR - http://www.scopus.com/inward/record.url?scp=77951970911&partnerID=8YFLogxK

U2 - 10.1016/j.ajog.2010.02.045

DO - 10.1016/j.ajog.2010.02.045

M3 - Article

AN - SCOPUS:77951970911

SN - 0002-9378

VL - 202

SP - 462.e1-462.e41

JO - American Journal of Obstetrics and Gynecology

JF - American Journal of Obstetrics and Gynecology

IS - 5

ER -

Characterization of the transcriptome of chorioamniotic membranes at the site of rupture in spontaneous labor at term

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this