Chemical chromatin ubiquitylation

Muhammad Jbara, Hao Sun, Guy Kamnesky, Ashraf Brik

Research output: Contribution to journalReview articlepeer-review

31 Scopus citations

Abstract

Histone modifications dynamically regulate chromatin structure and function, thereby mediating many processes that require access to DNA. Chemical protein synthesis has emerged as a powerful approach for generating homogeneously modified histone analogues in workable amounts for subsequent incorporation into nucleosome arrays for biochemical, functional and structural studies. This short review focuses on the strength of total chemical protein synthesis and semisynthetic approaches to generate ubiquitylated histones in their native or non-native forms and the utility of these analogues to decode the role of ubiquitylation in epigenetics.

Original languageEnglish
Pages (from-to)18-26
Number of pages9
JournalCurrent Opinion in Chemical Biology
Volume45
DOIs
StatePublished - 1 Aug 2018
Externally publishedYes

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry

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