Chemical Synthesis of Ubiquitin Chains

Hosahalli P. Hemantha, Somasekhar Bondalapati, Sumeet K. Singh, Ashraf Brik

Research output: Contribution to journalArticlepeer-review

1 Scopus citations


Chemical synthesis of complex biomolecules such as proteins is a challenging adventure, yet rewarding in driving various biochemical and biophysical research activities. Over the years, the refinement of peptide synthesis and invention of ligation methodologies have led to the successful synthesis of several complex protein targets. Ubiquitin bioconjugates, which are being studied intensively by many groups due to their involvement in numerous biological processes, represent a fine example where chemistry is greatly aiding these studies. In this article, we describe the synthetic routes and strategies to prepare different ubiquitin analogs with desired modifications, as well as di-ubiquitin chains.

Original languageEnglish
Pages (from-to)229-248
Number of pages20
JournalCurrent protocols in chemical biology
Issue number4
StatePublished - 1 Nov 2015
Externally publishedYes


  • SPPS
  • desulfurization
  • ligation
  • ubiquitin
  • ubiquitin chains

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology


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