Chemical synthesis of ubiquitinated peptides with varying lengths and types of ubiquitin chains to explore the activity of deubiquitinases

Sudhir N. Bavikar; Liat Spasser; Mahmood Haj-Yahya; Subramanian Vedhanarayanan Karthikeyan; Tal Moyal; K. S. Ajish Kumar; Ashraf Brik

doi:10.1002/anie.201106430

Chemical synthesis of ubiquitinated peptides with varying lengths and types of ubiquitin chains to explore the activity of deubiquitinases

Sudhir N. Bavikar, Liat Spasser, Mahmood Haj-Yahya, Subramanian Vedhanarayanan Karthikeyan, Tal Moyal, K. S. Ajish Kumar, Ashraf Brik

Department of Chemistry

Research output: Contribution to journal › Article › peer-review

77 Scopus citations

Abstract

Adding one at a time: A general and effective synthesis yields a peptide attached to mono-, di-, tri-, and tetraubiquitin (Ub) chains (see picture for peptides with Ub and Ub ₄), linked through lysine residues K48 or K63. These sets of ubiquitinated peptides were prepared in good quantities, and the activity of the enzymes UCH-L3 and IsoT with these different substrates was studied.

Original language	English
Pages (from-to)	758-763
Number of pages	6
Journal	Angewandte Chemie - International Edition
Volume	51
Issue number	3
DOIs	https://doi.org/10.1002/anie.201106430
State	Published - 16 Jan 2012

Keywords

peptide ligations
protein modifications
protein synthesis
solid-phase synthesis
ubiquitin

ASJC Scopus subject areas

Catalysis
General Chemistry

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10.1002/anie.201106430

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AU - Bavikar, Sudhir N.

AU - Spasser, Liat

AU - Haj-Yahya, Mahmood

AU - Karthikeyan, Subramanian Vedhanarayanan

AU - Moyal, Tal

AU - Ajish Kumar, K. S.

AU - Brik, Ashraf

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AB - Adding one at a time: A general and effective synthesis yields a peptide attached to mono-, di-, tri-, and tetraubiquitin (Ub) chains (see picture for peptides with Ub and Ub 4), linked through lysine residues K48 or K63. These sets of ubiquitinated peptides were prepared in good quantities, and the activity of the enzymes UCH-L3 and IsoT with these different substrates was studied.

KW - peptide ligations

KW - protein modifications

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Chemical synthesis of ubiquitinated peptides with varying lengths and types of ubiquitin chains to explore the activity of deubiquitinases

Abstract

Keywords

ASJC Scopus subject areas

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