Chemical synthesis of ubiquitinated peptides with varying lengths and types of ubiquitin chains to explore the activity of deubiquitinases

Sudhir N. Bavikar, Liat Spasser, Mahmood Haj-Yahya, Subramanian Vedhanarayanan Karthikeyan, Tal Moyal, K. S. Ajish Kumar, Ashraf Brik

Research output: Contribution to journalArticlepeer-review

71 Scopus citations

Abstract

Adding one at a time: A general and effective synthesis yields a peptide attached to mono-, di-, tri-, and tetraubiquitin (Ub) chains (see picture for peptides with Ub and Ub 4), linked through lysine residues K48 or K63. These sets of ubiquitinated peptides were prepared in good quantities, and the activity of the enzymes UCH-L3 and IsoT with these different substrates was studied.

Original languageEnglish
Pages (from-to)758-763
Number of pages6
JournalAngewandte Chemie - International Edition
Volume51
Issue number3
DOIs
StatePublished - 16 Jan 2012

Keywords

  • peptide ligations
  • protein modifications
  • protein synthesis
  • solid-phase synthesis
  • ubiquitin

ASJC Scopus subject areas

  • Catalysis
  • Chemistry (all)

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