Chloroplasts, Kinetin and Protein Synthesis


Research output: Contribution to journalArticlepeer-review

10 Scopus citations


The effect of kinetin on protein synthesis of isolated chloroplasts was investigated by following the incorporation of 14C‐leucine into isolated chloroplasts from Nicotiana tabacum. The incorporation activity varied greatly during the year, being largest in the winter and smallest in the summer. Conversely, the relative effect of kinetin on the incorporation of 14C‐leucine, whether applied as a pretreatment to the leaves or directly in the incubation medium, was largest in the summer and smallest or absent altogether in the winter. Kinetin did not prolong the net incorporation period, which lasted about 40 min, but only enhanced the initial rate of the reaction. Chloroplasts extracted from leaves that had been detached for 24 or 48 h displayed very little of their original, pre‐aged incorporation activity and treating the leaves with kinetin did not, essentially, prevent this loss. It was concluded that the major effect of kinetin upon chloroplasts may be related primarily to an effect upon hydration and permeability of the chloroplast and its membranes, and not to an effect directly upon its machinery for protein synthesis.

Original languageEnglish
Pages (from-to)176-180
Number of pages5
JournalPhysiologia Plantarum
Issue number2
StatePublished - 1 Jan 1971
Externally publishedYes

ASJC Scopus subject areas

  • Physiology
  • Genetics
  • Plant Science
  • Cell Biology


Dive into the research topics of 'Chloroplasts, Kinetin and Protein Synthesis'. Together they form a unique fingerprint.

Cite this