Chorioamnionitis and increased galectin-1 expression in PPROM - An anti-inflammatory response in the fetal membranes?

Nandor Gabor Than, Sung Su Kim, Asad Abbas, Yu Mi Han, John Hotra, Adi L. Tarca, Offer Erez, Derek E. Wildman, Juan Pedro Kusanovic, Beth Pineles, Daniel Montenegro, Samuel S. Edwin, Shali Mazaki-tovi, Francesca Gotsch, Jimmy Espinoza, Sonia S. Hassan, Zoltan Papp, Roberto Romero

Research output: Contribution to journalArticlepeer-review

25 Scopus citations

Abstract

Problem: Galectin-1 can regulate immune responses upon infection and inflammation. We determined galectin-1 expression in the chorioamniotic membranes and its changes during histological chorioamnionitis. Method of Study: Chorioamniotic membranes were obtained from women with normal pregnancy (n = 5) and from patients with pre-term pre-labor rupture of the membranes (PPROM) with (n = 8) and without histological chorioamnionitis (n = 8). Galectin-1 mRNA and protein were localized by in situ hybridization and immunohistochemistry. Galectin-1 mRNA expression was also determined by quantitative reverse transcriptase polymerase chain reaction. Rresults: Galectin-1 mRNA and protein were detected in the amniotic epithelium, chorioamniotic fibroblasts/myofibroblasts and macrophages, chorionic trophoblasts, and decidual stromal cells. In patients with PPROM, galectin-1 mRNA expression in the fetal membranes was higher (2.07-fold, P = 0.002) in those with chorioamnionitis than in those without. Moreover, chorioamionitis was associated with a strong galectin-1 immunostaining in amniotic epithelium, chorioamniotic mesodermal cells, and apoptotic bodies. Conclusion: Chorioamnionitis is associated with an increased galectin-1 mRNA expression and strong immunoreactivity of the chorioamniotic membranes; thus, galectin-1 may be involved in the regulation of the inflammatory responses to chorioamniotic infection.

Original languageEnglish
Pages (from-to)298-311
Number of pages14
JournalAmerican Journal of Reproductive Immunology
Volume60
Issue number4
DOIs
StatePublished - 18 Sep 2008
Externally publishedYes

Keywords

  • Chorioamniotic fibroblast/myofibroblast and macrophage
  • Chorioamniotic membranes
  • Inflammation
  • Lectin
  • Pre-labor rupture of membranes
  • Pre-term delivery

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