Circulating Cell-Free DNA Levels in Patients with Metastatic Renal Cell Carcinoma

Keren Rouvinov, Wilmosh Mermershtain, Hadas Dresler, Samuel Ariad, Reut Riff, Noa Shani-Shrem, Daniel Keizman, Amos Douvdevani

Research output: Contribution to journalArticlepeer-review

7 Scopus citations


Background: Limited data about biomarkers are available to predict the outcomes of targeted therapy in metastatic renal cell carcinoma (mRCC). Circulating cell-free DNA (CFD) is elevated in various cancers. Patients and Methods: We performed a prospective study of patients with mRCC who received targeted therapy in the Soroka Medical Center between 2013 and 2015. CFD levels were measured using a simple fluorometric assay. Blood samples for CFD were collected before treatment and at weeks 1, 4, 12, 18, and 24 of treatment. The normal cut-off level of CFD was defined as 800 ng/ml. The association of CFD with objective response, progression-free survival (PFS), and overall survival was tested, with adjustment for known confounding risk factors. Results: A total of 23 patients were included; 18 were treated with first-line therapy and 5 with second- and third-line therapies. Patients with normal pretreatment CFD level had a better PFS versus patients with increased levels (p = 0.023). In multivariate analysis, factors associated with PFS were pretreatment CFD levels (p = 0.020) and Heng risk (p = 0.006). Conclusions: Elevated pretreatment CFD levels measured using a simple fluorometric assay may be associated with a worse PFS in patients with mRCC. A larger prospective study is warranted in order to validate our observation.

Original languageEnglish
Pages (from-to)707-710
Number of pages4
JournalOncology Research and Treatment
Issue number11
StatePublished - 1 Oct 2017


  • Circulating cell-free DNA
  • Marker
  • Renal cell cancer
  • Response
  • Targeted therapy
  • Treatment

ASJC Scopus subject areas

  • Hematology
  • Oncology
  • Cancer Research


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