TY - JOUR
T1 - Clioquinol effects on tissue chelatable zinc in mice
AU - Nitzan, Yuval B.
AU - Sekler, Israel
AU - Frederickson, Christopher J.
AU - Coulter, Douglas A.
AU - Balaji, Rengarajan V.
AU - Liang, Shu Ling
AU - Margulis, Ariel
AU - Hershfinkel, Michal
AU - Silverman, William F.
N1 - Funding Information:
Acknowledgements We are grateful to Prof. Z. Ben-Zvi for expertise in applying the oral gavage technique. This work was supported by the German-Israel Binational Foundation (Grant #10588099) and the Israel Science Foundation (Grant #456) to I.S; The National Institutes of Health (Grants # NS042882, NS041682) to C.F.
PY - 2003/10/1
Y1 - 2003/10/1
N2 - Recent evidence for the involvement of zinc in the formation of β-amyloid plaques in the brain in Alzheimer's disease has led to the establishment of new therapeutic strategies for the degenerative disorder based on metal chelation. The present experiment was conducted on a membrane-permeable zinc chelator, clioquinol (CQ), that has shown potential in initial studies on a mouse model of Alzheimer's disease [1]. The degree of chelatable zinc in mice treated with CQ, delivered by two different routes, was measured using complementary protocols for identifying chelatable zinc: 6-methoxy-8-quinolyl-p-toluenesulfonamide (TSQ) histofluorescence, and selenite autometalography. Mice injected intraperitoneally with CQ showed a dramatic reduction in chelatable zinc in brain, testis, and pancreas. In contrast, mice given CQ orally showed no significant change in levels of chelatable zinc in these tissues. This suggests that CQ administered orally to patients with Alzheimer's disease should not significantly perturb chelatable zinc levels in key organs and may be used over long periods without adverse endocrinological and reproductive effects related to zinc deficiency. In contrast, CQ injected intraperitoneally may be used not only as a tool for investigating chelatable zinc pools but also in a clinical context. For example, injected CQ could be employed in situations requiring the rapid buffering of excessive chelatable zinc following ischemic episodes or brain trauma. Thus, our findings indicate that CQ has considerable potential as a versatile scientific and clinical tool used for selective modulation of zinc pools.
AB - Recent evidence for the involvement of zinc in the formation of β-amyloid plaques in the brain in Alzheimer's disease has led to the establishment of new therapeutic strategies for the degenerative disorder based on metal chelation. The present experiment was conducted on a membrane-permeable zinc chelator, clioquinol (CQ), that has shown potential in initial studies on a mouse model of Alzheimer's disease [1]. The degree of chelatable zinc in mice treated with CQ, delivered by two different routes, was measured using complementary protocols for identifying chelatable zinc: 6-methoxy-8-quinolyl-p-toluenesulfonamide (TSQ) histofluorescence, and selenite autometalography. Mice injected intraperitoneally with CQ showed a dramatic reduction in chelatable zinc in brain, testis, and pancreas. In contrast, mice given CQ orally showed no significant change in levels of chelatable zinc in these tissues. This suggests that CQ administered orally to patients with Alzheimer's disease should not significantly perturb chelatable zinc levels in key organs and may be used over long periods without adverse endocrinological and reproductive effects related to zinc deficiency. In contrast, CQ injected intraperitoneally may be used not only as a tool for investigating chelatable zinc pools but also in a clinical context. For example, injected CQ could be employed in situations requiring the rapid buffering of excessive chelatable zinc following ischemic episodes or brain trauma. Thus, our findings indicate that CQ has considerable potential as a versatile scientific and clinical tool used for selective modulation of zinc pools.
KW - Autometallography
KW - Metal
KW - Zinc
KW - Zinc metabolism
KW - Zinc pools
UR - http://www.scopus.com/inward/record.url?scp=0242288463&partnerID=8YFLogxK
U2 - 10.1007/s00109-003-0462-7
DO - 10.1007/s00109-003-0462-7
M3 - Article
C2 - 12928783
AN - SCOPUS:0242288463
SN - 0946-2716
VL - 81
SP - 637
EP - 644
JO - Journal of Molecular Medicine
JF - Journal of Molecular Medicine
IS - 10
ER -