Abstract
The herbicide norflurazon inhibits carotene biosynthesis in photosynthetic organisms by blocking the enzyme phytoene dehydrogenase (= phytoene desaturase). We have isolated norflurazon-resistant mutants of the cyanobacterium Synechococcus PCC7942. The herbicide-resistance gene from the mutant NFZ4 has been cloned by genetic complementation of the resistance trait in wild type cells. The experiment described here illustrates the usefulness of employing cyanobacteria to clone herbicide-resistance genes in a quick and simple way.
Original language | English |
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Pages (from-to) | 482-486 |
Journal | Zeitschrift fur Naturforschung - Section C Journal of Biosciences |
Volume | 45 |
Issue number | 5 |
DOIs | |
State | Published - 1 Jan 1990 |
Externally published | Yes |
Keywords
- Cyanobacteria
- Gene Cloning
- Herbicide Resistance
- Norflurazon
- Phytoene Dehydrogenase (= Phytoene Desaturase)
ASJC Scopus subject areas
- General Biochemistry, Genetics and Molecular Biology