Cloning a Gene Coding for Norflurazon Resistance in Cyanobacteria

Daniel Chamovitz, Iris Pecker, Gerhard Sandmann, Peter Böger, Joseph Hirschberg

Research output: Contribution to journalArticlepeer-review

31 Scopus citations

Abstract

The herbicide norflurazon inhibits carotene biosynthesis in photosynthetic organisms by blocking the enzyme phytoene dehydrogenase (= phytoene desaturase). We have isolated norflurazon-resistant mutants of the cyanobacterium Synechococcus PCC7942. The herbicide-resistance gene from the mutant NFZ4 has been cloned by genetic complementation of the resistance trait in wild type cells. The experiment described here illustrates the usefulness of employing cyanobacteria to clone herbicide-resistance genes in a quick and simple way.

Original languageEnglish
Pages (from-to)482-486
JournalZeitschrift fur Naturforschung - Section C Journal of Biosciences
Volume45
Issue number5
DOIs
StatePublished - 1 Jan 1990
Externally publishedYes

Keywords

  • Cyanobacteria
  • Gene Cloning
  • Herbicide Resistance
  • Norflurazon
  • Phytoene Dehydrogenase (= Phytoene Desaturase)

ASJC Scopus subject areas

  • General Biochemistry, Genetics and Molecular Biology

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