Cloning and Characterization of Acetohydroxyacid Synthase from Bacillus stearothermophilus

Iris Porat, Michael Vinogradov, Maria Vyazmensky, Chung Dar Lu, David M. Chipman, Ahmed T. Abdelal, Ze'ev Barak

Research output: Contribution to journalArticlepeer-review

24 Scopus citations


Five genes from the ilv-leu operon from Bacillus stearothermophilus have been sequenced. Acetohydroxyacid synthase (AHAS) and its subunits were separately cloned, purified, and characterized. This thermophilic enzyme resembles AHAS III of Escherichia coli, and regulatory subunits of AHAS III complement the catalytic subunit of the AHAS of B. stearothermophilus, suggesting that AHAS III is functionally and evolutionally related to the single AHAS of gram-positive bacteria.

Original languageEnglish
Pages (from-to)570-574
Number of pages5
JournalJournal of Bacteriology
Issue number2
StatePublished - 1 Jan 2004

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology


Dive into the research topics of 'Cloning and Characterization of Acetohydroxyacid Synthase from Bacillus stearothermophilus'. Together they form a unique fingerprint.

Cite this