Cloning and Characterization of Acetohydroxyacid Synthase from Bacillus stearothermophilus

Iris Porat; Michael Vinogradov; Maria Vyazmensky; Chung Dar Lu; David M. Chipman; Ahmed T. Abdelal; Ze'ev Barak

doi:10.1128/JB.186.2.570-574.2004

Cloning and Characterization of Acetohydroxyacid Synthase from Bacillus stearothermophilus

Iris Porat, Michael Vinogradov, Maria Vyazmensky, Chung Dar Lu, David M. Chipman, Ahmed T. Abdelal, Ze'ev Barak

Department of Life Sciences

Research output: Contribution to journal › Article › peer-review

24 Scopus citations

Abstract

Five genes from the ilv-leu operon from Bacillus stearothermophilus have been sequenced. Acetohydroxyacid synthase (AHAS) and its subunits were separately cloned, purified, and characterized. This thermophilic enzyme resembles AHAS III of Escherichia coli, and regulatory subunits of AHAS III complement the catalytic subunit of the AHAS of B. stearothermophilus, suggesting that AHAS III is functionally and evolutionally related to the single AHAS of gram-positive bacteria.

Original language	English
Pages (from-to)	570-574
Number of pages	5
Journal	Journal of Bacteriology
Volume	186
Issue number	2
DOIs	https://doi.org/10.1128/JB.186.2.570-574.2004
State	Published - 1 Jan 2004

ASJC Scopus subject areas

Microbiology
Molecular Biology

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10.1128/JB.186.2.570-574.2004

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abstract = "Five genes from the ilv-leu operon from Bacillus stearothermophilus have been sequenced. Acetohydroxyacid synthase (AHAS) and its subunits were separately cloned, purified, and characterized. This thermophilic enzyme resembles AHAS III of Escherichia coli, and regulatory subunits of AHAS III complement the catalytic subunit of the AHAS of B. stearothermophilus, suggesting that AHAS III is functionally and evolutionally related to the single AHAS of gram-positive bacteria.",

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T1 - Cloning and Characterization of Acetohydroxyacid Synthase from Bacillus stearothermophilus

AU - Porat, Iris

AU - Vinogradov, Michael

AU - Vyazmensky, Maria

AU - Lu, Chung Dar

AU - Chipman, David M.

AU - Abdelal, Ahmed T.

AU - Barak, Ze'ev

PY - 2004/1/1

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N2 - Five genes from the ilv-leu operon from Bacillus stearothermophilus have been sequenced. Acetohydroxyacid synthase (AHAS) and its subunits were separately cloned, purified, and characterized. This thermophilic enzyme resembles AHAS III of Escherichia coli, and regulatory subunits of AHAS III complement the catalytic subunit of the AHAS of B. stearothermophilus, suggesting that AHAS III is functionally and evolutionally related to the single AHAS of gram-positive bacteria.

AB - Five genes from the ilv-leu operon from Bacillus stearothermophilus have been sequenced. Acetohydroxyacid synthase (AHAS) and its subunits were separately cloned, purified, and characterized. This thermophilic enzyme resembles AHAS III of Escherichia coli, and regulatory subunits of AHAS III complement the catalytic subunit of the AHAS of B. stearothermophilus, suggesting that AHAS III is functionally and evolutionally related to the single AHAS of gram-positive bacteria.

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Cloning and Characterization of Acetohydroxyacid Synthase from Bacillus stearothermophilus

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