Cloning and characterization of the δ6 polyunsaturated fatty acid elongase from the green microalga Parietochloris incisa

Umidjon Iskandarov; Inna Khozin-Goldberg; Rivka Ofir; Zvi Cohen

doi:10.1007/s11745-009-3301-y

Cloning and characterization of the δ6 polyunsaturated fatty acid elongase from the green microalga Parietochloris incisa

Umidjon Iskandarov, Inna Khozin-Goldberg, Rivka Ofir, Zvi Cohen

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24 Scopus citations

Abstract

The very-long-chain polyunsaturated fatty acid (VLC-PUFA), arachidonic acid (ARA, 20:4ω-6) is a component of neuron tissues such as brain and retina cells and a primary substrate for the biosynthesis of biologically active eicosanoids. The green freshwater microalga Parietochloris incisa (Trebouxiophyceae) has been shown to accumulate an extraordinary high content of ARA-rich triacylglycerols. It was thus interesting to characterize the genes involved in lipid biosynthesis in this alga. We report here the identification of a cDNA encoding for a P. incisa PUFA elongase (PiELO1) and demonstrate that the expression of PiELO1 in yeast Saccharomyces cereviseae confers its elongase activity on C18 δ6 PUFA. Phylogenetic analysis indicated that PiELO1 is highly similar to functionally characterized δ6 PUFA elongase genes from other green algae and lower plants. Quantitative real-time PCR expression studies showed that PiELO1 is upregulated under nitrogen starvation, the condition triggering and enhancing storage oil and ARA accumulation in P. incisa.

Original language	English
Pages (from-to)	545-554
Number of pages	10
Journal	Lipids
Volume	44
Issue number	6
DOIs	https://doi.org/10.1007/s11745-009-3301-y
State	Published - 1 Jun 2009

Keywords

Arachidonic acid
Fatty acid elongation
Nitrogen starvation
Parietochloris incisa
Subtractive hybridization
VLC-PUFA

ASJC Scopus subject areas

Biochemistry
Organic Chemistry
Cell Biology

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title = "Cloning and characterization of the δ6 polyunsaturated fatty acid elongase from the green microalga Parietochloris incisa",

abstract = "The very-long-chain polyunsaturated fatty acid (VLC-PUFA), arachidonic acid (ARA, 20:4ω-6) is a component of neuron tissues such as brain and retina cells and a primary substrate for the biosynthesis of biologically active eicosanoids. The green freshwater microalga Parietochloris incisa (Trebouxiophyceae) has been shown to accumulate an extraordinary high content of ARA-rich triacylglycerols. It was thus interesting to characterize the genes involved in lipid biosynthesis in this alga. We report here the identification of a cDNA encoding for a P. incisa PUFA elongase (PiELO1) and demonstrate that the expression of PiELO1 in yeast Saccharomyces cereviseae confers its elongase activity on C18 δ6 PUFA. Phylogenetic analysis indicated that PiELO1 is highly similar to functionally characterized δ6 PUFA elongase genes from other green algae and lower plants. Quantitative real-time PCR expression studies showed that PiELO1 is upregulated under nitrogen starvation, the condition triggering and enhancing storage oil and ARA accumulation in P. incisa.",

keywords = "Arachidonic acid, Fatty acid elongation, Nitrogen starvation, Parietochloris incisa, Subtractive hybridization, VLC-PUFA",

author = "Umidjon Iskandarov and Inna Khozin-Goldberg and Rivka Ofir and Zvi Cohen",

note = "Funding Information: Acknowledgments This work was supported by a grant from the Israeli ministry of science, culture and sport.",

year = "2009",

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doi = "10.1007/s11745-009-3301-y",

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T1 - Cloning and characterization of the δ6 polyunsaturated fatty acid elongase from the green microalga Parietochloris incisa

AU - Iskandarov, Umidjon

AU - Khozin-Goldberg, Inna

AU - Ofir, Rivka

AU - Cohen, Zvi

N1 - Funding Information: Acknowledgments This work was supported by a grant from the Israeli ministry of science, culture and sport.

PY - 2009/6/1

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N2 - The very-long-chain polyunsaturated fatty acid (VLC-PUFA), arachidonic acid (ARA, 20:4ω-6) is a component of neuron tissues such as brain and retina cells and a primary substrate for the biosynthesis of biologically active eicosanoids. The green freshwater microalga Parietochloris incisa (Trebouxiophyceae) has been shown to accumulate an extraordinary high content of ARA-rich triacylglycerols. It was thus interesting to characterize the genes involved in lipid biosynthesis in this alga. We report here the identification of a cDNA encoding for a P. incisa PUFA elongase (PiELO1) and demonstrate that the expression of PiELO1 in yeast Saccharomyces cereviseae confers its elongase activity on C18 δ6 PUFA. Phylogenetic analysis indicated that PiELO1 is highly similar to functionally characterized δ6 PUFA elongase genes from other green algae and lower plants. Quantitative real-time PCR expression studies showed that PiELO1 is upregulated under nitrogen starvation, the condition triggering and enhancing storage oil and ARA accumulation in P. incisa.

AB - The very-long-chain polyunsaturated fatty acid (VLC-PUFA), arachidonic acid (ARA, 20:4ω-6) is a component of neuron tissues such as brain and retina cells and a primary substrate for the biosynthesis of biologically active eicosanoids. The green freshwater microalga Parietochloris incisa (Trebouxiophyceae) has been shown to accumulate an extraordinary high content of ARA-rich triacylglycerols. It was thus interesting to characterize the genes involved in lipid biosynthesis in this alga. We report here the identification of a cDNA encoding for a P. incisa PUFA elongase (PiELO1) and demonstrate that the expression of PiELO1 in yeast Saccharomyces cereviseae confers its elongase activity on C18 δ6 PUFA. Phylogenetic analysis indicated that PiELO1 is highly similar to functionally characterized δ6 PUFA elongase genes from other green algae and lower plants. Quantitative real-time PCR expression studies showed that PiELO1 is upregulated under nitrogen starvation, the condition triggering and enhancing storage oil and ARA accumulation in P. incisa.

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KW - Nitrogen starvation

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Cloning and characterization of the δ6 polyunsaturated fatty acid elongase from the green microalga Parietochloris incisa

Abstract

Keywords

ASJC Scopus subject areas

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