Cloning and molecular characterization of the salt-regulated jojoba ScRab cDNA encoding a small GTP-binding protein

Ela Mizrahi-Aviv; David Mills; Aliza Benzioni; Dudy Bar-Zvi

doi:10.1080/1042517021000014967

Cloning and molecular characterization of the salt-regulated jojoba ScRab cDNA encoding a small GTP-binding protein

Ela Mizrahi-Aviv, David Mills, Aliza Benzioni, Dudy Bar-Zvi

Department of Life Sciences

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

Salt stress results in a massive change in gene expression. An 837 bp cDNA designated ScRab was cloned from shoot cultures of the salt tolerant jojoba (Simmondsia chinesis). The cloned cDNA encodes a full length 200 amino acid long polypeptide that bears high homology to the Rab subfamily of small GTP binding proteins, particularly, the Rab5 subfamily. ScRab expression is reduced in shoots grown in the presence of salt compared to shoots from non-stressed cultures. His 6 -tagged ScRAB protein was expressed in E. coli, and purified to homogeneity. The purified protein bound radiolabelled GTP. The unlabelled guanine nucleotides GTP, GTP γ S and GDP but not ATP, CTP or UTP competed with GTP binding.

Original language	English
Pages (from-to)	295-300
Number of pages	6
Journal	Mitochondrial DNA
Volume	13
Issue number	5
DOIs	https://doi.org/10.1080/1042517021000014967
State	Published - 1 Dec 2002

Keywords

Differential display
GTP-binding protein
Jojoba
Rab
Salinity
Simmondsia chinesis

ASJC Scopus subject areas

Molecular Biology
Genetics

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1080/1042517021000014967

Cite this

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title = "Cloning and molecular characterization of the salt-regulated jojoba ScRab cDNA encoding a small GTP-binding protein",

abstract = "Salt stress results in a massive change in gene expression. An 837 bp cDNA designated ScRab was cloned from shoot cultures of the salt tolerant jojoba (Simmondsia chinesis). The cloned cDNA encodes a full length 200 amino acid long polypeptide that bears high homology to the Rab subfamily of small GTP binding proteins, particularly, the Rab5 subfamily. ScRab expression is reduced in shoots grown in the presence of salt compared to shoots from non-stressed cultures. His 6 -tagged ScRAB protein was expressed in E. coli, and purified to homogeneity. The purified protein bound radiolabelled GTP. The unlabelled guanine nucleotides GTP, GTP γ S and GDP but not ATP, CTP or UTP competed with GTP binding.",

keywords = "Differential display, GTP-binding protein, Jojoba, Rab, Salinity, Simmondsia chinesis",

author = "Ela Mizrahi-Aviv and David Mills and Aliza Benzioni and Dudy Bar-Zvi",

note = "Funding Information: This research was funded in part by a grant from the Chief Scientist of The Israel Ministry of Agriculture. We thank Tova Trebitsch and Jerry Eichler for critical reading of the manuscript.",

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T1 - Cloning and molecular characterization of the salt-regulated jojoba ScRab cDNA encoding a small GTP-binding protein

AU - Mizrahi-Aviv, Ela

AU - Mills, David

AU - Benzioni, Aliza

AU - Bar-Zvi, Dudy

N1 - Funding Information: This research was funded in part by a grant from the Chief Scientist of The Israel Ministry of Agriculture. We thank Tova Trebitsch and Jerry Eichler for critical reading of the manuscript.

PY - 2002/12/1

Y1 - 2002/12/1

N2 - Salt stress results in a massive change in gene expression. An 837 bp cDNA designated ScRab was cloned from shoot cultures of the salt tolerant jojoba (Simmondsia chinesis). The cloned cDNA encodes a full length 200 amino acid long polypeptide that bears high homology to the Rab subfamily of small GTP binding proteins, particularly, the Rab5 subfamily. ScRab expression is reduced in shoots grown in the presence of salt compared to shoots from non-stressed cultures. His 6 -tagged ScRAB protein was expressed in E. coli, and purified to homogeneity. The purified protein bound radiolabelled GTP. The unlabelled guanine nucleotides GTP, GTP γ S and GDP but not ATP, CTP or UTP competed with GTP binding.

AB - Salt stress results in a massive change in gene expression. An 837 bp cDNA designated ScRab was cloned from shoot cultures of the salt tolerant jojoba (Simmondsia chinesis). The cloned cDNA encodes a full length 200 amino acid long polypeptide that bears high homology to the Rab subfamily of small GTP binding proteins, particularly, the Rab5 subfamily. ScRab expression is reduced in shoots grown in the presence of salt compared to shoots from non-stressed cultures. His 6 -tagged ScRAB protein was expressed in E. coli, and purified to homogeneity. The purified protein bound radiolabelled GTP. The unlabelled guanine nucleotides GTP, GTP γ S and GDP but not ATP, CTP or UTP competed with GTP binding.

KW - Differential display

KW - GTP-binding protein

KW - Jojoba

KW - Rab

KW - Salinity

KW - Simmondsia chinesis

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U2 - 10.1080/1042517021000014967

DO - 10.1080/1042517021000014967

M3 - Article

AN - SCOPUS:0036806252

SN - 1940-1736

VL - 13

SP - 295

EP - 300

JO - Mitochondrial DNA

JF - Mitochondrial DNA

IS - 5

ER -

Cloning and molecular characterization of the salt-regulated jojoba ScRab cDNA encoding a small GTP-binding protein

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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