Cloning and molecular characterization of the salt-regulated jojoba ScRab cDNA encoding a small GTP-binding protein

Ela Mizrahi-Aviv, David Mills, Aliza Benzioni, Dudy Bar-Zvi

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

Salt stress results in a massive change in gene expression. An 837 bp cDNA designated ScRab was cloned from shoot cultures of the salt tolerant jojoba (Simmondsia chinesis). The cloned cDNA encodes a full length 200 amino acid long polypeptide that bears high homology to the Rab subfamily of small GTP binding proteins, particularly, the Rab5 subfamily. ScRab expression is reduced in shoots grown in the presence of salt compared to shoots from non-stressed cultures. His 6 -tagged ScRAB protein was expressed in E. coli, and purified to homogeneity. The purified protein bound radiolabelled GTP. The unlabelled guanine nucleotides GTP, GTP γ S and GDP but not ATP, CTP or UTP competed with GTP binding.

Original languageEnglish
Pages (from-to)295-300
Number of pages6
JournalMitochondrial DNA
Volume13
Issue number5
DOIs
StatePublished - 1 Dec 2002

Keywords

  • Differential display
  • GTP-binding protein
  • Jojoba
  • Rab
  • Salinity
  • Simmondsia chinesis

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