Cloning of NaCl-regulated genes from cultures of jojoba nodal segments by differential display RT-PCR

Ela Mizrahi, Michal Gura, Dudy Bar-Zvi, David Mills, Aliza Benzioni

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

1 Scopus citations

Abstract

Jojoba (Simmondsia chinesis) is a salt tolerant evergreen perennial shrub native to the Sonoran Desert. We are using Differential Display Reverse Transcriptase Polymerase Chain Reaction (DDRT-PCR) to isolate NaCl regulated genes from jojoba variety 64, by comparing genes expressed in nodal segments grown in cultures in the absence or ? presence of NaCl. We have isolated several cDNA clones representing genes whose expression is either increased or decreased by the salt treatment.

Original languageEnglish
Title of host publicationIII International Symposium on In Vitro Culture and Horticultural Breeding
PublisherInternational Society for Horticultural Science
Pages461-463
Number of pages3
ISBN (Print)9789066059092
DOIs
StatePublished - 1 Jan 1997

Publication series

NameActa Horticulturae
Volume447
ISSN (Print)0567-7572

Keywords

  • DDRT-PCR
  • Gene expression
  • Salt-stress
  • Salt-tolerance

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